semiquinone glucoside derivative provides protection to male reproductive system of the mice against gamma radiation toxicity

• Published in: Environmental toxicology 2014-05, Vol.29 (5), p.558-567
• Main Authors: Patel, Dev Dutt, Bansal, Deen Dayal, Mishra, Saurabh, Arora, Rajesh, Sharma, Rakesh Kumar, Jain, Swatantra Kumar, Kumar, Raj
• Format: Article
• Language: English
• Subjects: Animals; Bacillus; Bacillus (bacteria); Bacillus - chemistry; bcl-2-Associated X Protein - metabolism; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; gamma radiation; Gamma Rays - adverse effects; germ cells; Glucosides - pharmacology; ionizing radiation; irradiation; Male; Mice; Mice, Inbred BALB C; NF-kappa B - metabolism; protein synthesis; Proto-Oncogene Proteins c-bcl-2 - metabolism; Quinones - pharmacology; Radiation-Protective Agents - pharmacology; radioprotection; reproductive system; seminiferous tubules; semiquinone glucoside derivatives (SQGD); Testis - drug effects; Testis - pathology; Testis - radiation effects; Time Factors; toxicity; Tumor Suppressor Protein p53 - metabolism; Whole-Body Irradiation - adverse effects
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.21781

Present investigation was carried out to evaluate the radioprotective efficacy of a novel Semiquinone glucoside derivative (SQGD), isolated from Bacillus sp. INM‐1, in the male reproductive system of BALB/c mice. Animals were administered 50 mg/kg b.wt. (i.p.) SQGD 2 h before whole body γ‐irradiation (10 Gy). Radiation‐induced cellular toxicity and its modulation by SQGD pretreatment was evaluated in the mice testes by quantitative histological and protein expression analysis. SQGD pretreatment protects irradiated mice from radiation‐induced testicular atrophy and germ cells degeneration, which may lead to emptiness of seminiferous tubules. Significant decrease in P53 and P21⁽Cⁱᵖ/ᵂᴬF‐¹⁾ expression was observed in the irradiated mice pretreated (2 h) by SQGD at 6 h compared with only irradiated mice. However, contrary to P53, expressions of P21 at latter time, that is, 24–72 h was found to be increased significantly in the irradiated mice pretreated by SQGD. Significant increase in the intact PARP‐1 protein expression were observed in the testes of the mice pretreated by SQGD 2 h before irradiation at 24–72 h compared with the only irradiated mice, whereas significant increase in PARP‐1 cleaved fragment was noticed at 24 h. Similarly, significant increase in NF‐kB and BCL‐2/BAX expressions ratio was noticed in SQGD‐treated mice (± irradiation) compared with irradiated mice, suggested a role of SQGD in the activation of prosurvival signaling in the testicular germinal cells population of the irradiated mice and thus contributed to protection against lethal γ‐irradiation. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 558–567, 2014.