Permeability Effects on the Efficiency of Antioxidant Nanoreactors

Enzyme-loaded polymeric vesicles, or polymersomes, can be regarded as nanoreactors, which, for example, can be applied as artificial organelles. We implemented a naturally occurring enzymatic cascade reaction in two types of polymersomes, which are known to possess different permeability properties....

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Bibliographic Details
Published in:Biomacromolecules 2013-07, Vol.14 (7), p.2364-2372
Main Authors: Louzao, Iria, van Hest, Jan C. M
Format: Article
Language:English
Subjects:
Antioxidants - metabolism
Applied sciences
Artificial Cells - metabolism
Biological and medical sciences
Biological Transport
Bioreactors
Biotechnology
Catalase - chemistry
Catalase - metabolism
Cell Membrane
Enzyme Assays
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Organic polymers
Oxidative Stress
Oxygen - chemistry
Oxygen - metabolism
Permeability
Physicochemistry of polymers
Polymers - chemistry
Properties and characterization
Reactive Oxygen Species - metabolism
Special properties (catalyst, reagent or carrier)
Superoxide Dismutase - chemistry
Superoxide Dismutase - metabolism
Superoxide Dismutase-1
Superoxides
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Summary:Enzyme-loaded polymeric vesicles, or polymersomes, can be regarded as nanoreactors, which, for example, can be applied as artificial organelles. We implemented a naturally occurring enzymatic cascade reaction in two types of polymersomes, which are known to possess different permeability properties. The selected cascade reaction involved the antioxidant enzymes superoxide dismutase (SOD1) and catalase (CAT) for combating oxidative stress. The activities of both enzymes were investigated by spectrophotometric and electrochemical assays. Whereas the SOD1 substrate (the radical anion superoxide, O2 •–) was able to penetrate both membranes equally well, the CAT substrate (H2O2) showed different rates of diffusion. When O2 •– was generated inside polymersomes filled with both SOD1 and CAT, the activities of the two systems were comparable again.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm400493b