Antifungal and antimycotoxigenic metabolites in Anacardiaceae species from northwest Argentina: isolation, identification and potential for control of Fusarium species

• Published in: Journal of applied microbiology 2014-05, Vol.116 (5), p.1262-1273
• Main Authors: Aristimuño Ficoseco, M.E, Vattuone, M.A, Audenaert, K, Catalán, C.A.N, Sampietro, D.A
• Format: Article
• Language: English
• Subjects: alkylcatechols; Anacardiaceae; Anacardiaceae - metabolism; antifungal agents; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; antifungal properties; Argentina; Biological and medical sciences; biosynthesis; Coumaric Acids - pharmacology; deoxynivalenol; ear rot; ethyl acetate; ferulic acid; food preservatives; fumonisins; Fumonisins - metabolism; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium - drug effects; Fusarium - growth & development; Fusarium graminearum; Fusarium verticillioides; leaves; lipids; lupeol; Metabolites; methanol; methylene chloride; microbial growth; Microbiology; minimum inhibitory concentration; Mycotoxins - biosynthesis; Pentacyclic Triterpenes - pharmacology; Plant diseases; Plant Extracts - pharmacology; Plant pathology; Plant resistance; Schinopsis; Schinus; Trichothecenes - biosynthesis
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12436

AIMS: The purpose of this research was to identify antifungal compounds from leaves of Schinus and Schinopsis species useful for the control of toxigenic Fusarium species responsible of ear rot diseases. METHODS AND RESULTS: Leaves of Schinopsis (S. lorentzii and S. haenkeana) and Schinus (S. areira, S. gracilipes and S. fasciculatus) were sequentially extracted with dichloromethane, ethyl acetate and methanol. The antifungal activity of the fraction soluble in methanol of these extracts (fCH₂Cl₂, fAcEt and fMeOH, respectively) was determined by the broth microdilution method and the disc‐diffusion method. The minimum inhibitory dose (MID), the diameter of growth inhibition (DGI) and the minimum concentration for 50% inhibition of fungal growth (MIC₅₀) were calculated. The fCH₂Cl₂and fAcEt of the Schinopsis species had the lowest MID and MIC₅₀values and the highest DGI. The antifungal compounds were identified as lupeol and a mix of phenolic lipids. The last one had the highest antifungal activity with MIC₅₀31–28 μg g⁻¹and 165–150 μg g⁻¹on Fusarium graminearum and Fusarium verticillioides, respectively. The identified metabolites completely inhibited fumonisin and deoxynivalenol production at lower concentrations than ferulic acid, a natural antimycotoxigenic compound. CONCLUSIONS: It was proven that lupeol and phenolic lipids were inhibitors of both fungal growth and mycotoxin production of toxigenic Fusarium species. This fact is specially interesting in the control of the toxigenic Fusarium species because several commercial antifungals showed to stimulate mycotoxin biosynthesis at sublethal concentrations. SIGNIFICANCE AND IMPACT OF THE STUDY: Control of toxigenic Fusarium species requires compounds able to inhibit both fungal growth and mycotoxin production. Our results suggest that the use of lupeol as food preservative and the phenolic lipids as fungal growth inhibitors of F. verticillioides and F. graminearum did not imply an increase in mycotoxin accumulation.