Zinc increases phorbol ester receptors in intact B-cells, neutrophil polymorphs and platelets

• Published in: FEBS letters 1989-04, Vol.247 (2), p.445-447
• Main Authors: Forbes, I.J., Zalewski, P.D., Hurst, N.P., Giannakis, C., Whitehouse, M.W.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; B-cell, Neutrophil, Platelet; B-Lymphocytes - drug effects; B-Lymphocytes - metabolism; Biological and medical sciences; Blood Platelets - drug effects; Blood Platelets - metabolism; Caenorhabditis elegans Proteins; Carrier Proteins; CLL, chronic lymphatic leukaemia; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Humans; Kinetics; Leukemia, Lymphocytic, Chronic, B-Cell - metabolism; Neutrophils - drug effects; Neutrophils - metabolism; PBS, phosphate-buffered saline; PDBu, phorbol dibutyrate; Phenanthrolines - pharmacology; Phorbol 12,13-Dibutyrate - metabolism; PKC, protein kinase C; Protein kinase C; Protein Kinase C - metabolism; Pyridines - pharmacology; Receptors, Drug - drug effects; Receptors, Drug - metabolism; Thiones; Transferases; Tumor Cells, Cultured; Zinc; Zinc - pharmacology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(89)81388-2

In the presence of pyrithione, which was used as a Zn 2+ ionophore, Zn 2+ (10–100 μM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10 6 cells), neutrophil polymorphs (0.2 to 0.51 pmol/10 6 cells) and platelets (91 to 209 pmol/10 10 cells). Fractionation of cells after treatment with Zn pyrithione showed that increased binding of PDBu occurred in the particulate fraction of cells and this was accompanied by loss of phorbol ester receptors from the cytosol. These data are compatible with a role for Zn in the subcellular distribution and activation of protein kinase C.