Molecular characterization and xenogenic application of wharton’s jelly derived caprine mesenchymal stem cells

Aim of the present study was in vitro expansion and characterization of caprine wharton’s jelly derived mesenchymal stem cells (cWJ-MSCs) to investigate their tissue healing potential in xenogenic animal model. Plastic adherent fibroblastoid cell populations with distinctive homogeneous morphology w...

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Bibliographic Details
Published in:Veterinary research communications 2014-06, Vol.38 (2), p.139-148
Main Authors: Pratheesh, M. D., Gade, Nitin E., Dubey, Pawan K., Nath, Amar, Sivanarayanan, T. B., Madhu, D. N., Sharma, Bhaskar, Amarpal, Saikumar, G., Sharma, G. Taru
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - metabolism
Biomedical and Life Sciences
Cell Differentiation
Cell Proliferation
Cells, Cultured
Goats
Heterografts - cytology
Life Sciences
Male
Mesenchymal Stromal Cells - cytology
Original Article
Rabbits
Random Allocation
Veterinary Medicine/Veterinary Science
Wound Healing
Zoology
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Summary:Aim of the present study was in vitro expansion and characterization of caprine wharton’s jelly derived mesenchymal stem cells (cWJ-MSCs) to investigate their tissue healing potential in xenogenic animal model. Plastic adherent fibroblastoid cell populations with distinctive homogeneous morphology were isolated from caprine wharton’s jelly explants. These wharton’s jelly derived cells were found positive for the surface markers CD-73, STRO-1 and CD-105, whereas they were negative for hematopoetic stem cell marker CD-34. In vitro cultured cWJ-MSCs also showed differentiation properties into osteogenic, adipogenic and chondrogenic lineages as demonstrated by von Kossa, Oil Red- O and Alcian blue staining respectively, which was further confirmed and quantified by flow cytometric analysis. Furthermore, these well characterized cWJ-MSCs were evaluated for the wound-healing potential in full-thickness skin wounds in rabbit model for 28 days. Caprine WJ- MSCs treated skin wounds showed significantly ( P  
ISSN:0165-7380
1573-7446
DOI:10.1007/s11259-014-9597-y