Separation of proteins by microcolumn liquid chromatography based on the reversed-phase and size-exclusion principles

Slurry-packed fused-silica microcolumns of 250 μm I.D., are characterized for use in high-performance liquid chromatographic studies of proteins. The present work utilizes the reversed-phase and size-exclusion chromatographic modes for the separation of standard protein mixtures. A 5-μm, 300-Å octyl...

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Bibliographic Details
Published in:Journal of Chromatography A 1988, Vol.448 (1), p.73-86
Main Authors: Flurer, Cheryl L., Borra, Claudio, Andreolini, Franca, Novotny, Milos
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
liquid chromatography
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Summary:Slurry-packed fused-silica microcolumns of 250 μm I.D., are characterized for use in high-performance liquid chromatographic studies of proteins. The present work utilizes the reversed-phase and size-exclusion chromatographic modes for the separation of standard protein mixtures. A 5-μm, 300-Å octyl material is utilized for the reversed-phase studies, and the size-exclusion studies are accomplished with 5-μm diol material of 60-, 100- and 300-Å pore sizes. Column efficiency and packing reproducibility, as well as sample capacity in a micropreparative mode, are discussed. In addition, the inherent mass sensitivity of a microcolumn liquid chromatography system as applied to protein detection is demonstrated.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)84566-0