IL-27 improves migrational and antiviral potential of CB dendritic cells

Abstract Interleukin (IL)-27 is known to be increased considerably in cord blood (CB) dendritic cells (DCs) after TLR ligation. Previously, we demonstrated that also basal IL-27 levels are higher in CB DCs. Here, we examined effects of IL-27 on monocyte derived dendritic cells (moDCs) to approach it...

Full description

Saved in:
Bibliographic Details
Published in:Human immunology 2014-06, Vol.75 (6), p.584-591
Main Authors: Birkholz, Julia, Doganci, Aysefa, Darstein, Claudia, Gehring, Stephan, Zepp, Fred, Meyer, Claudius U
Format: Article
Language:English
Subjects:
Adult
Allergy and Immunology
Cell Differentiation
Cell Movement - drug effects
Cells, Cultured
Chemokine CXCL10 - genetics
Chemokine CXCL10 - metabolism
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - metabolism
Fetal Blood - cytology
Fetal Blood - metabolism
Gene Expression Regulation
Humans
Infant, Newborn
Interferon Regulatory Factors - genetics
Interferon Regulatory Factors - metabolism
Interleukin-8 - genetics
Interleukin-8 - metabolism
Interleukins - biosynthesis
Interleukins - genetics
Interleukins - pharmacology
Monocytes - cytology
Monocytes - metabolism
Receptors, CCR1 - genetics
Receptors, CCR1 - metabolism
Signal Transduction
STAT1 Transcription Factor - genetics
STAT1 Transcription Factor - metabolism
Transcription, Genetic
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Interleukin (IL)-27 is known to be increased considerably in cord blood (CB) dendritic cells (DCs) after TLR ligation. Previously, we demonstrated that also basal IL-27 levels are higher in CB DCs. Here, we examined effects of IL-27 on monocyte derived dendritic cells (moDCs) to approach its particular role in the specialized immune system of the human neonate. Exogenous IL-27 promotes IL-27 transcription in CB and adult blood (AB) moDCs. IL-27 acts on CB moDCs primarily by significantly augmenting IL-27 protein, secondarily by increasing transcription of CXCL10 among other chemokines, chemokine receptor CCR1, interferon stimulated genes, transcription factor IRF8 and genes involved in antigen presentation. Furthermore, CB moDCs respond to IL-27 with augmented IL-8 and Tumor necrosis factor (TNF)-α. The results suggest that IL-27 enhances migrational and antiviral properties of CB dendritic cells.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2014.02.004