Aprotinin reduces oxidative stress induced by pneumoperitoneum in rats

Abstract Background Ischemia–reperfusion injury induced by pneumoperitoneum is a well-studied entity, which increases oxidative stress during laparoscopic operations. The reported anti-inflammatory action of aprotinin was measured in a pneumoperitoneum model in rats for the first time in this study....

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Bibliographic Details
Published in:The Journal of surgical research 2014-06, Vol.189 (2), p.238-248
Main Authors: Baltatzis, Minas, MD, Pavlidis, Theodoros E., MD, PhD, Ouroumidis, Odysseas, MD, Koliakos, Georgios, MD, PhD, Nikolaidou, Christina, MD, Venizelos, Ioannis, MD, PhD, Michopoulou, Anna, MD, Sakantamis, Athanasios, MD, PhD
Format: Article
Language:English
Subjects:
Animals
Aprotinin
Aprotinin - drug effects
Aprotinin - therapeutic use
Ischemia–reperfusion injury
Liver - pathology
Male
Oxidative stress
Pneumoperitoneum
Pneumoperitoneum, Artificial - adverse effects
Protein Carbonylation - drug effects
Random Allocation
Rats
Rats, Wistar
Reperfusion Injury - etiology
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Serine Proteinase Inhibitors - pharmacology
Serine Proteinase Inhibitors - therapeutic use
Splanchnic Circulation
Surgery
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Summary:Abstract Background Ischemia–reperfusion injury induced by pneumoperitoneum is a well-studied entity, which increases oxidative stress during laparoscopic operations. The reported anti-inflammatory action of aprotinin was measured in a pneumoperitoneum model in rats for the first time in this study. Materials and methods A total of 60 male Albino Wistar rats were used in our protocol. Prolonged pneumoperitoneum (4 h) was applied, causing splanchnic ischemia and a period of reperfusion with a duration of 60 or 180 min followed. Several cytokines and markers of oxidative stress were measured in liver, small intestine, and lungs to compare the aprotinin group with the control group. Tissue inflammation was also evaluated and compared between groups using a five-scaled histopathologic score. Results In aprotinin group values of biochemical markers (tumor necrosis factor α, interleukin 6, endothelin 1, C reactive protein, pro-oxidant–antioxidant balance, and carbonyl proteins) were lower in all tissues studied. Statistical significance was greater in liver and lungs ( P  
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2014.02.036