Aspirin Decreases Systemic Exposure to Clopidogrel Through Modulation of P-Glycoprotein But Does Not Alter Its Antithrombotic Activity

• Published in: Clinical pharmacology and therapeutics 2014-06, Vol.95 (6), p.608-616
• Main Authors: Oh, J, Shin, D, Lim, K S, Lee, S, Jung, K-H, Chu, K, Hong, K S, Shin, K-H, Cho, J-Y, Yoon, S H, Ji, S C, Yu, K-S, Lee, H, Jang, I-J
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate - pharmacology; Adult; Area Under Curve; Aryl Hydrocarbon Hydroxylases - genetics; Aryl Hydrocarbon Hydroxylases - metabolism; Aryldialkylphosphatase - genetics; Aryldialkylphosphatase - metabolism; Aspirin - pharmacology; ATP-Binding Cassette, Sub-Family B, Member 1 - drug effects; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Biological and medical sciences; Cytochrome P-450 CYP2C19; Drug Interactions; Fibrinolytic Agents - pharmacology; Genotype; Humans; Male; Medical sciences; Pharmacology. Drug treatments; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - pharmacokinetics; Platelet Aggregation Inhibitors - pharmacology; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Ticlopidine - analogs & derivatives; Ticlopidine - pharmacokinetics; Ticlopidine - pharmacology
• ISSN: 0009-9236; 1532-6535
• DOI: 10.1038/clpt.2014.49

Decreased oral clopidogrel absorption caused by induction of intestinal permeability glycoprotein (P‐gp) expression after aspirin administration was observed in rats. This study evaluated the effect of aspirin coadministration on the pharmacokinetics/pharmacodynamics of clopidogrel in humans. A single 75‐mg dose of clopidogrel was orally administered before and after 2 and 4 weeks of once‐daily 100‐mg aspirin administration in 18 healthy volunteers who were recruited based on CYP2C19 and PON1 genotypes. Plasma concentrations of clopidogrel and its active metabolite, H4, and relative platelet inhibition (RPI) were determined. The P‐gp microRNA miR‐27a increased by up to 7.67‐fold (P = 0.004) and the clopidogrel area under the concentration–time curve (AUC) decreased by 14% (P > 0.05), but the AUC of H4 remained unchanged and RPI increased by up to 15% (P = 0.002) after aspirin administration. These findings indicate low‐dose aspirin coadministration may decrease clopidogrel bioavailability but does not decrease its efficacy. Clinical Pharmacology & Therapeutics (2014); 95 6, 608–616. doi:10.1038/clpt.2014.49