Fe(III)-TAML activator: a potent peroxidase mimic for chemiluminescent determination of hydrogen peroxide

• Published in: Talanta (Oxford) 2014-07, Vol.125, p.361-365
• Main Authors: Vdovenko, Marina M, Demiyanova, Alexandra S, Kopylov, Kirill E, Sakharov, Ivan Yu
• Format: Article
• Language: English
• Subjects: Biosensing Techniques; Calibration; Catalysis; Dose-Response Relationship, Drug; Hemin - chemistry; Hydrogen Peroxide - analysis; Hydrogen Peroxide - chemistry; Hydrogen-Ion Concentration; Ligands; Luminescence; Luminol - chemistry; Oxygen - chemistry; Peroxidases - chemistry; Reproducibility of Results
• ISSN: 1873-3573
• DOI: 10.1016/j.talanta.2014.03.040

Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercially available Fe(III)-TAML activator 1a, which was shown to be a more active catalyst than hemin. At Fe(III)-TAML activator 1a use in chemiluminescent assay for H2O2 determination the detection limit value (3σ) of 5×10(-8)M was similar to the detection limit obtained with horseradish peroxidase (1×10(-7)M) and significantly lower than that obtained in the presence of hemin (6×10(-7)M). The linear ranges (R(2)=0.98) of the assay were 6×10(-8)-1×10(-6)M and 6×10(-7)-1×10(-6)M H2O2 for Fe(III)-TAML 1a and hemin, respectively. The CV values for Fe(III)-TAML 1a-based assay measured within the working range varied from 1.0% to 3.7% (n=4), whereas in the case of hemin -5.0% to 9.7% (n=4). Moreover, the sensitivity of Fe(III)-TAML 1a-based method was 56 and 5 times higher than that of hemin- and HRP-based methods, respectively. The obtained results open good perspectives to apply Fe(III)-TAML activator 1a in CL analytical methods instead of hemin, a traditionally used peroxidase mimetic.