Rapid decolorization of methyl orange by a novel Aeromonas sp. strain DH-6

Azo dyes are extensively used, but are recalcitrant and refractory. In this study, an indigenous strain DH-6 was isolated and identified as Aeromonas sp. based on 16S rDNA analysis for its excellent methyl orange (MO) decolorizing capability. Plackett-Burman design and response surface methodology (...

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Bibliographic Details
Published in:Water science and technology 2014-01, Vol.69 (10), p.2004-2013
Main Authors: DU, Lin-Na, GANG LI, XU, Fang-Cheng, XIU PAN, WEN, Ling-Ning, YANG WANG
Format: Article
Language:English
Subjects:
Aeromonas - genetics
Aeromonas - metabolism
Analysis methods
Applied sciences
Azo Compounds - chemistry
Azo Compounds - metabolism
Azo dyes
Bioremediation
Confidence intervals
Decoloring
Dyes
Exact sciences and technology
General purification processes
Glucose
High temperature
Industrial Waste
Natural water pollution
Optimization
Phylogeny
Pollution
Response surface methodology
Textile Industry
Waste Disposal, Fluid
Wastewaters
Water Pollutants, Chemical - chemistry
Water Pollutants, Chemical - metabolism
Water treatment and pollution
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Summary:Azo dyes are extensively used, but are recalcitrant and refractory. In this study, an indigenous strain DH-6 was isolated and identified as Aeromonas sp. based on 16S rDNA analysis for its excellent methyl orange (MO) decolorizing capability. Plackett-Burman design and response surface methodology (RSM) were employed to investigate the effect of operational parameters on decolorization and to optimize the decolorization process. Based on the results the concentrations of glucose, Na2HPO4 and MO and temperature were selected as the four significant parameters of RSM. The optimal conditions for MO decolorization by the strain were as follows: 3.0 g/L glucose, 4.9 g/L Na2HPO4, 100 mg/L MO, and at 40 °C. The verification tests showed that 95.5% decolorization was observed after incubation for 2 h, which is within the confidence interval. Under the optimal conditions, the kinetics of the decolorization fitted the first-order model well (R(2) = 0.969). As the strain DH-6 still showed a good decolorizing capability at a relatively high temperature, it is considered a candidate for azo dye bioremediation in some tropical or subtropical regions.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2014.099