The pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp. lycopersici

Abstract Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing Pseudomonas kilonensis. In this study, the mutant JX22MT1 was obtained by the EZ-Tn5 transposon mutation and showed no antifungal activity against Fusarium oxys...

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Bibliographic Details
Published in:FEMS microbiology letters 2014-04, Vol.353 (2), p.98-105
Main Authors: Xu, Jianhong, Deng, Peng, Showmaker, Kurt C., Wang, Hui, Baird, Sonya M., Lu, Shi-En
Format: Article
Language:English
Subjects:
Antibiosis
Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
antimicrobial activity
bacteria
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Bacterial Proteins - pharmacology
Base Sequence
Cloning, Molecular
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
fungi
Fusarium - drug effects
Fusarium - growth & development
Fusarium oxysporum
Microbiology
Molecular Sequence Data
mutagenesis
Mutagenesis, Insertional
Phylogeny
Pseudomonas
Pseudomonas - genetics
Pseudomonas - isolation & purification
Pseudomonas - metabolism
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:Abstract Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing Pseudomonas kilonensis. In this study, the mutant JX22MT1 was obtained by the EZ-Tn5 transposon mutation and showed no antifungal activity against Fusarium oxysporum f. sp. lycopersici as compared with wild-type strain JX22. The pqqC gene was disrupted in the mutant. Antifungal activity at the wild-type level was restored from the mutant JX22MT1 with the introduction of the functional pqqC gene, which encodes pyrroloquinoline–quinone synthesis protein C. The results suggest that pqqC is essential for antifungal activity of P. kilonensis JX22 against F. oxysporum f. sp. lycopersici. The pqqC gene, which is widely distributed in many bacteria and encodes pyrroloquinoline quinine (PQQ), is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum.
ISSN:0378-1097
1574-6968
DOI:10.1111/1574-6968.12411