Stimulation of polyphosphoinositide hydrolysis in Swiss 3T3 cells by recombinant fibroblast growth factors

• Published in: FEBS letters 1989-04, Vol.247 (2), p.227-231
• Main Authors: Brown, Kenneth D., Blakeley, Diana M., Brigstock, David R.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Bombesin; Bombesin - pharmacology; Ca 2+ mobilization; Ca2+ mobilization; Calcium - metabolism; Cell Line; DNA - biosynthesis; Dose-Response Relationship, Drug; Drug Synergism; FGF, fibroblast growth factor; Fibroblast growth factor; Fibroblast Growth Factors - pharmacology; fibroblasts; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Hydrolysis; Inositol - metabolism; Inositol Phosphates - metabolism; IPs, inositol phosphates; Kinetics; PBS, phosphate-buffered saline; Phosphatidylinositol turnover; Phosphatidylinositols - metabolism; phosphoinositides; Protein hormones. Growth factors. Cytokines; Proteins; Recombinant Proteins - pharmacology; Tetradecanoylphorbol Acetate - pharmacology; TPA, 12-O-tetradecanoyl-phorbol-13-acetate; Vasopressins - pharmacology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(89)81340-7

Mitogenic concentrations of recombinant acidic or basic fibroblast growth factor (FGF) stimulated the accumulation of [ 3H]inositol phosphates ([ 3H]IPs) in Swiss 3T3 cells pre-labelled for 48 h with [ 3H]inositol. Maximal effects were obtained at 0.3 ng/ml and 3 ng/ml for basic and acidic FGF, respectively. Higher doses of either factor led to a diminished stimulation. FGF also stimulated 45Ca 2+ release from cells pre-labelled with the isotope. However, FGF-stimulated production of [ 3H]IPs and release of 45Ca 2+ exhibited marked differences when compared with the responses to the peptide mitogen bombesin; the FGF responses were markedly slower and were not inhibited by phorbol esters.