Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori

Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid d...

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Bibliographic Details
Published in:Genetics and molecular research 2014-01, Vol.13 (2), p.3747-3760
Main Authors: Chen, Q M, Cheng, D J, Liu, S P, Ma, Z G, Tan, X, Zhao, P
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bombyx - enzymology
Bombyx - genetics
Cloning, Molecular
Expressed Sequence Tags
Fatty Acid Desaturases - biosynthesis
Fatty Acid Desaturases - genetics
Fatty Acids - metabolism
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Genome, Insect
Multigene Family - genetics
Phylogeny
Sequence Alignment
Stearoyl-CoA Desaturase - biosynthesis
Stearoyl-CoA Desaturase - genetics
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Summary:Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven genes contain 3 conserved histidine cluster motifs and 4 transmembrane domains, but their N-terminal residues exhibit obvious diversity. Phylogenetic analysis revealed that there are 6 groups; Bmdesat1 and Bmdesat5-8 were clustered into group 2, which is involved in Δ11 desaturation activity, and Bmdesat3-4 were grouped in group 1, which is involved in Δ9 desaturation activity. Twelve of the 14 genes have expressed sequence tag evidence. Microarray data and reverse transcription polymerase chain reaction analysis demonstrated that Bmdesat3-4 and Bmdesat10 were expressed from the larval to moth stages and in multiple tissues on day 3 of 5th instar larvae. Bmdesat9, Bmdesat11, and Bmdesat14 were expressed during the pupal and late-embryonic stage, suggesting that they may take part in fatty acid metabolism to provide energy. These results provide some insights into the functions of individual fatty acid desaturases in silkworm.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.May.13.2