Honokiol suppresses TNF-α-induced migration and matrix metalloproteinase expression by blocking NF-κB activation via the ERK signaling pathway in rat aortic smooth muscle cells

• Published in: Acta histochemica 2014-05, Vol.116 (4), p.588-595
• Main Authors: Zhu, Xiaoying, Wang, Zhansheng, Hu, Cuizhu, Li, Zhao, Hu, Jian
• Format: Article
• Language: English
• Subjects: Animals; Aorta - drug effects; Biphenyl Compounds - pharmacology; ERK; Honokiol; Lignans - pharmacology; Male; MAP Kinase Signaling System - drug effects; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinase-2/9; Migration; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; NF-kappa B - drug effects; NF-kappa B - metabolism; Nuclear factor-κB; Rats, Sprague-Dawley; Signal Transduction - drug effects; Tumor Necrosis Factor-alpha - metabolism; Vascular smooth muscle cells
• ISSN: 0065-1281; 1618-0372
• DOI: 10.1016/j.acthis.2013.11.005

Honokiol, a small-molecule polyphenol derived and isolated from the Chinese medicinal herb Magnolia officinalis, has been shown to possess a wide range of pharmacological activities. In the present study, we aimed to investigate the effects of honokiol on tumor necrosis factor-α (TNF-α)-induced migration in rat aortic smooth muscle cells (RASMCs). We found that honokiol inhibited TNF-α-induced RASMC proliferation and migration in a dose-dependent manner. At the molecular level, pretreatment with honokiol blocked TNF-α-induced protein expression of matrix metalloproteinase (MMP)-2 and MMP-9, nuclear factor (NF)-κB activation, and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. Moreover, NF-κB inhibitor (BAY 11-7028) and ERK inhibitor (U0126) also mimicked the inhibitory effects of honokiol in TNF-α-treated RASMCs. In conclusion, these results indicate that honokiol suppresses TNF-α-induced migration and MMP expression by blocking NF-κB activation via the ERK signaling pathway in RASMCs. Our findings support honokiol as a promising novel agent for the prevention and treatment of atherosclerosis.