Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis

Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 prein...

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Bibliographic Details
Published in:The Journal of infectious diseases 1989-07, Vol.160 (1), p.95-103
Main Authors: Wong, Brian, Brauer, Karen L., Tsai, Ring R., Jayasimhulu, Koka
Format: Article
Language:English
Subjects:
Animals
Aspergillosis
Aspergillosis - diagnosis
Aspergillosis - metabolism
Aspergillus
Aspergillus fumigatus - metabolism
Biological and medical sciences
Chromatography, Gas
Conidia
Creatinine - blood
Gas Chromatography-Mass Spectrometry
Homogenization
Human mycoses
Infections
Infectious diseases
Kidney - microbiology
Kidneys
Liver
Liver - analysis
Liver - microbiology
Liver Diseases - diagnosis
Liver Diseases - metabolism
Lung - microbiology
Major Articles
Male
Mannitol - analysis
Mannitol - blood
Medical sciences
Metabolic diseases
Mycoses
Mycoses of the respiratory system
Rats
Rats, Inbred Strains
Spleen - microbiology
Tissue samples
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Summary:Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 preincubated conidia of Aspergillus jumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be d-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more d-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P < .01 at 12, 24, and 36 h) and higher serum d-mannitol concentrations and serum d-mannitol/creatinine ratios after 36 h (P < .05) than did uninfected controls. These results indicate that A. jumigatus can produce and release sufficient d-mannitol in the tissues of infected animals to raise serum d-mannitol levels. Thus, d-mannitol is a potential diagnostic marker for aspergillosis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/160.1.95