Cloned Endothelial Cells from Fetal Bovine Bone

Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibite...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (3), p.916-920
Main Authors: Streeten, Elizabeth A., Ornberg, Richard, Curcio, Francesco, Sakaguchi, Kazushige, Marx, Stephen, Aurbach, Gerald D., Brandi, Maria Luisa
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Bone and Bones - cytology
Bone marrow
Bones
Cattle
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell Division - drug effects
Cell growth
Cell lines
Cells
Cells, Cultured
Clone Cells
Cultured cells
Cyclic AMP - metabolism
DNA Replication - drug effects
Endothelial cells
Endothelium - cytology
Endothelium - drug effects
Fetus
Fundamental and applied biological sciences. Psychology
Growth Substances - pharmacology
Heparin - pharmacology
Human umbilical vein endothelial cells
Kinetics
Molecular and cellular biology
Ungulates
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.3.916