Cloned Endothelial Cells from Fetal Bovine Bone

• Published in: Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (3), p.916-920
• Main Authors: Streeten, Elizabeth A., Ornberg, Richard, Curcio, Francesco, Sakaguchi, Kazushige, Marx, Stephen, Aurbach, Gerald D., Brandi, Maria Luisa
• Format: Article
• Language: English
• Subjects: Animal cells; Animals; Biological and medical sciences; Bone and Bones - cytology; Bone marrow; Bones; Cattle; Cell culture techniques; Cell cultures. Hybridization. Fusion; Cell Division - drug effects; Cell growth; Cell lines; Cells; Cells, Cultured; Clone Cells; Cultured cells; Cyclic AMP - metabolism; DNA Replication - drug effects; Endothelial cells; Endothelium - cytology; Endothelium - drug effects; Fetus; Fundamental and applied biological sciences. Psychology; Growth Substances - pharmacology; Heparin - pharmacology; Human umbilical vein endothelial cells; Kinetics; Molecular and cellular biology; Ungulates
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.86.3.916

Primary cell cultures from fetal bovine sternum were developed in Coon's modified Ham's F-12 medium containing 10% Nu-Serum, 1% Ultroser-G, and 200 mg of galactose per liter. Clones were obtained by colony isolation; one clone, BBE-1, was selected for characterization. BBE-1 cells exhibited typical endothelial morphology by light and electron microscopy and immunofluorescence for factor VIII-related antigen throughout their life span of 8 months. The cells showed mitogenic responses to endothelial cell growth factor, basic fibroblast growth factor, insulin-like growth factor types I and II, platelet-derived growth factor, ascorbic acid, and progesterone. Parathyroid hormone stimulated intracellular accumulation of cAMP in BBE-1 cells but not in endothelial cells from two other tissues. These clonal cells provide a useful system for studies on bone vasculature, including its interactions with other bone cells.