Ca super(2+) oscillations induced by hormonal stimulation of individual fura-2-loaded hepatocytes

Isolated rat hepatocytes were loaded with the Ca super(2+) indicator fura-2 to measure cytosolic free Ca super(2+) concentrations ((Ca super(2+)) sub(i)) in individual cells by digital ratio imaging microscopy. Reduction in extracellular (Ca super(2+)) decreased the frequency but not amplitude of th...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-01, Vol.264 (22), p.12859-12866
Main Authors: Kawanishi, T, Blank, L M, Harootunian, A T, Smith, M T, Tsien, R Y
Format: Article
Language:English
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Summary:Isolated rat hepatocytes were loaded with the Ca super(2+) indicator fura-2 to measure cytosolic free Ca super(2+) concentrations ((Ca super(2+)) sub(i)) in individual cells by digital ratio imaging microscopy. Reduction in extracellular (Ca super(2+)) decreased the frequency but not amplitude of the spikes, suggesting that the spikes result from dumping of intracellular stores and that the entry of extracellular Ca super(2+) affects only the rate of replenishment of those stores.
ISSN:0021-9258