Inhibition of fluorescent advanced glycation end products (AGEs) of human serum albumin upon incubation with 3-β-hydroxybutyrate

• Published in: Molecular biology reports 2014-06, Vol.41 (6), p.3705-3713
• Main Authors: Bohlooli, M, Moosavi-Movahedi, A. A, Taghavi, F, Saboury, A. A, Maghami, P, Seyedarabi, A, Moosavi-Movahedi, F, Ahmad, F, Shockravi, A, Habibi-Rezaei, M
• Format: Article
• Language: English
• Subjects: 3-Hydroxybutyric Acid - administration & dosage; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Calorimetry, Differential Scanning; Circular Dichroism; Diabetes Complications - metabolism; Diabetes Complications - pathology; differential scanning calorimetry; enthalpy; Exercise - physiology; fasting; fluorescence; glucose; Glucose - metabolism; glycation; Glycation End Products, Advanced - antagonists & inhibitors; Glycation End Products, Advanced - chemistry; Glycation End Products, Advanced - metabolism; Glycosylation - drug effects; Histology; human serum albumin; Humans; Life Sciences; liver; Morphology; neurodegenerative diseases; patients; Serum Albumin - drug effects; Serum Albumin - metabolism; Spectrometry, Fluorescence; spectroscopy; Thermodynamics
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3235-1

Advanced glycation end products (AGEs), which are the final products of glycation, have a major role in diabetic complication and neurodegenerative disorders. The 3-β-hydroxybutyrate (3BHB), a ketone body which is produced by the liver, can be detected in increased concentrations in individuals post fasting and prolonged exercises and in diabetic (type I) patients. In this study, the inhibitory effect of 3BHB on AGEs formation by glucose from the human serum albumin (HSA) was studied at physiological conditions after 35 days of incubation, using physical techniques such as circular dichroism and fluorescence spectroscopy, as well as differential scanning calorimetry (DSC). The fluorescence intensity measurements of glycated HSA by glucose (GHSA) in the presence of 3BHB indicate a decrease in AGEs formation. The DSC deconvolution profile results also confirm the protective role of 3BHB on incubated with glucose by preventing the enthalpy reduction of the HSA tail segment, compared with the deconvolution profile seen for incubated with glucose alone. The concentration of 3BHB used in this study is in accordance with the concentration detected in the body of individuals post fasting and prolonged exercises.