Phylogeographical analysis of Ligia oceanica (Crustacea: Isopoda) reveals two deeply divergent mitochondrial lineages

Isopods of the species Ligia oceanica are typical inhabitants of the rocky intertidal of the northern European coastline. The aim of this study was to assess the genetic structure of this species using mitochondrial and nuclear sequence data. We analysed partial mitochondrial cytochrome c oxidase su...

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Bibliographic Details
Published in:Biological journal of the Linnean Society 2014-05, Vol.112 (1), p.16-30
Main Authors: Raupach, Michael J, Bininda‐Emonds, Olaf R. P, Knebelsberger, Thomas, Laakmann, Silke, Pfaender, Jobst, Leese, Florian
Format: Article
Language:English
Subjects:
16S rRNA gene
coasts
Crustacea
cytochrome c oxidase subunit I
cytochrome-c oxidase
genes
glacial refugia
haplotypes
homogenization
Isopoda
Ligia
Ligia oceanica
long-distance dispersal
mitochondrial DNA
nucleotide sequences
Oniscidea
phylogeography
ribosomal RNA
Wolbachia
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Summary:Isopods of the species Ligia oceanica are typical inhabitants of the rocky intertidal of the northern European coastline. The aim of this study was to assess the genetic structure of this species using mitochondrial and nuclear sequence data. We analysed partial mitochondrial cytochrome c oxidase subunit I (CO1) and 16S rRNA gene sequence data of 161 specimens collected from ten sites ranging from Spain to Norway. For selected specimens, we also sequenced the hypervariable V7 expansion segment of the nuclear 18S rRNA gene as a supplementary marker. Furthermore, we studied the infection rate of all analysed specimens by the alphaproteobacterium Wolbachia. Our analyses revealed two deeply divergent mitochondrial lineages for Ligia oceanica that probably diverged in the late Pliocene to mid Pleistocene. One lineage comprised specimens from northern populations (‘lineage N’) and one primarily those from France and Spain (‘lineage S’). Distribution patterns of the haplotypes and the genetic distances between both lineages revealed two populations that diverged before the Last Glacial Maximum. Given that we found no homogenization of mitochondrial haplotypes, our present results also reject any influence of Wolbachia on the observed mtDNA variability. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 112, 16–30.
ISSN:0024-4066
1095-8312
DOI:10.1111/bij.12254