In vivo immunogenicity of Tax(11–19) epitope in HLA-A2/DTR transgenic mice: Implication for dendritic cell-based anti-HTLV-1 vaccine

• Published in: Vaccine 2014-05, Vol.32 (26), p.3274-3284
• Main Authors: Sagar, Divya, Masih, Shet, Schell, Todd, Jacobson, Steven, Comber, Joseph D, Philip, Ramila, Wigdahl, Brian, Jain, Pooja, Khan, Zafar K
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Animals; Applied microbiology; Biological and medical sciences; CD11c-DTR transgenic mice; CD8-Positive T-Lymphocytes - immunology; Cytokines; Dendritic cells; Dendritic Cells - immunology; Female; Fundamental and applied biological sciences. Psychology; Gene Products, tax - immunology; HLA-A2 Antigen - immunology; HLA-A2.1 transgenic mice; HTLV-1; HTLV-I Infections - prevention & control; Human T-lymphotropic virus; Human T-lymphotropic virus 1; Immune response; Immune system; Immunodominant Epitopes - immunology; Immunogenicity; Infections; Interleukin-6 - blood; Leukemia; Male; Mice, Inbred C57BL; Mice, Transgenic; Microbiology; Miscellaneous; Peptides; Proteins; Rodents; Tax; Transforming Growth Factor beta - blood; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral Vaccines - immunology; Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2014.03.087

Highlights • HLA-A2/DTR transgenic mouse strain was generated to study immunogenicity of Tax(11–19) epitope. • A reduced proliferation of CD8+ splenocytes from Tax(11–19) immunized DC-depleted mice is observed. • Adjuvant usage demonstrated higher frequency of Tax(11–19)-specific cells. • Tax(11–19) epitope can be a potential candidate for a DC-based anti-HTLV-1 vaccine.