Biological and genetic diversity of Wheat yellow mosaic virus (Genus Bymovirus)

• Published in: Phytopathology 2014-03, Vol.104 (3), p.313-319
• Main Authors: Ohki, Takehiro, Netsu, Osamu, Kojima, Hisayo, Sakai, Junichi, Onuki, Masatoshi, Maoka, Tetsuo, Shirako, Yukio, Sasaya, Takahide
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Bymovirus; Genetic Variation; Genome, Viral - genetics; Genotype; Geography; Japan; Molecular Sequence Data; Phylogeny; Plant Diseases - virology; Polymorphism, Restriction Fragment Length; Potyviridae - classification; Potyviridae - genetics; Potyviridae - isolation & purification; RNA, Viral - genetics; Sequence Analysis, RNA; Triticum - virology; Triticum aestivum; Wheat yellow mosaic virus
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-06-13-0150-R

The biological and genetic diversity of Wheat yellow mosaic virus (WYMV) isolates in Japan was characterized. On the basis of wheat cultivar reactions, 14 WYMV isolates from various places were classified into pathotypes I, II, or III. These were distributed in central, northern, and southern areas of Japan, respectively. WYMV isolates comprised three genotypes (A, A' and B) based on amino acid differences in RNA1 and two genotypes (a and b) based on amino acid differences in RNA2. A correlation was found between the WYMV RNA1-based genotype and pathotype, suggesting that factors associated with pathogenicity map to RNA1. Genotype Aa and A'a were distributed mainly in the central to southern areas of Japan, and genotype Bb was found in northern areas of Japan, as shown by reverse-transcription polymerase chain reaction restriction fragment length polymorphism analysis. Chinese isolates YA and YZ were closely related to genotypes Bb and Aa, respectively. Wheat was introduced from China to Japan in the 4th and 5th centuries, and the two genotypes of WYMV might also have been introduced with the crop from China and later adapted to local wheat cultivars in Japan.