Burning of a narrow spectral hole at 1.7 K in the absorbance band of the primary electron donor of Rhodopseudomonas viridis reaction centers with blocked electron transfer

• Published in: FEBS letters 1988-09, Vol.237 (1), p.57-60
• Main Authors: Shuvalov, V.A., Klevanik, A.V., Ganago, A.O., Shkuropatov, A.Ya, Gubanov, V.S.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell structures and functions; Chloroplast, photosynthetic membrane and photosynthesis; Electron transfer; Fundamental and applied biological sciences. Psychology; Hole burning; Molecular and cellular biology; Primary electron donor; Reaction center; Rhodopseudomonas viridis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(88)80171-6

Burning of spectral holes within the absorption band of the primary electron donor P in reaction centers of Rhodopseudomonas viridis is studied using selective excitation at 1014 nm and 1.7 K. A narrow (≤ 1.0 cm −1) spectral hole with relatively weak electron-phonon and vibronic coupling is burned if the intermediary electron acceptor I is pre-reduced. The hole is broadened up to ∼90–150 cm −1 if I is neutral and the electron-transfer chain is open. Broadening of the hole is discussed in terms of the fast (∼70–110 fs) electron hopping between P* and P +BL − (BL, bacteriochlorophyll monomer in L protein subunit).