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Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment
Purpose To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial grow...
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| Published in: | Graefe's archive for clinical and experimental ophthalmology 2014-06, Vol.252 (6), p.881-888 |
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| container_title | Graefe's archive for clinical and experimental ophthalmology |
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| creator | Loukovaara, Sirpa Lehti, Kaisa Robciuc, Alexandra Pessi, Timo Holopainen, Juha M. Koli, Katri Immonen, Ilkka Keski-Oja, Jorma |
| description | Purpose
To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker.
Methods
Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker (control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography.
Results
The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all
p
|
| doi_str_mv | 10.1007/s00417-013-2508-z |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1530320347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1530320347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-5aaa35b0435e5ef0f79df6259107cc24d7791ac2eb9e1ef92523c095c6dfe3da3</originalsourceid><addsrcrecordid>eNp1kM1KAzEYRYMoWqsP4EYG3LiJfkkmprMU8adQcKPQlSHNfNOmzE9N0op9elNaRQRXIeTcm8sh5IzBFQNQ1wEgZ4oCE5RLGND1HumxXEiqgI_3SQ8UZ3Qg-PiIHIcwh4QLyQ7JEc85G6Rsj7wNW-vRBCwz10ZvVi6ma52Zduq6RecwupbyrMYV1iEzIXTWmZjoDxdnmZ_htDGxm2LbLUPmN3QKlxiNnTXYxhNyUJk64Onu7JPXh_uXuyc6en4c3t2OqBWKRyqNMUJONvNQYgWVKsrqhsuCgbKW56VSBTOW46RAhlXBJRcWCmlvygpFaUSfXG57F757X2KIunHBYl2bFtMyzaQAwUHkKqEXf9B5t_Rp9o5ikBcsUWxLWd-F4LHSC-8a4z81A72Rr7fydZKvN_L1OmXOd83LSYPlT-LbdgL4FgjpqZ2i__X1v61fw-qRVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1530310491</pqid></control><display><type>article</type><title>Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment</title><source>Springer Link</source><creator>Loukovaara, Sirpa ; Lehti, Kaisa ; Robciuc, Alexandra ; Pessi, Timo ; Holopainen, Juha M. ; Koli, Katri ; Immonen, Ilkka ; Keski-Oja, Jorma</creator><creatorcontrib>Loukovaara, Sirpa ; Lehti, Kaisa ; Robciuc, Alexandra ; Pessi, Timo ; Holopainen, Juha M. ; Koli, Katri ; Immonen, Ilkka ; Keski-Oja, Jorma</creatorcontrib><description>Purpose
To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker.
Methods
Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker (control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography.
Results
The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all
p
< 0.01), whereas concentration of VEGF was lower (
p
= 0.01) in eyes with RRD relative to controls. Logistic regression analysis identified Ang-2 concentration as a novel marker of RRD (
p
= 0.0001, OR 48.7). Ang-1, EPO, and total TGF-β1 levels were not significantly different between the groups. However, TGF-β1 and MMP-2 were increased in eyes with total RRD compared to those with local RRD (
p
≤ 0.05). In eyes with PVR, no differences were observed in any studied marker as compared with non-PVR eyes.
Conclusions
Current results reveal Ang-2 as a key factor upregulated in RRD. It may co-operate with fibrosis-associated factors and contribute to vascular complications such as breakdown of blood–eye barrier and PVR development.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-013-2508-z</identifier><identifier>PMID: 24218041</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aged ; Angiopoietin-1 - metabolism ; Angiopoietin-2 - metabolism ; Biomarkers - metabolism ; Enzyme-Linked Immunosorbent Assay ; Erythropoietin - metabolism ; Female ; Humans ; Male ; Matrix Metalloproteinases - metabolism ; Medicine ; Medicine & Public Health ; Middle Aged ; Ophthalmology ; Prospective Studies ; Retinal Detachment - diagnosis ; Retinal Detachment - metabolism ; Retinal Disorders ; Transforming Growth Factor beta1 - metabolism ; Vascular Endothelial Growth Factor A - metabolism ; Vitreous Body - metabolism</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2014-06, Vol.252 (6), p.881-888</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-5aaa35b0435e5ef0f79df6259107cc24d7791ac2eb9e1ef92523c095c6dfe3da3</citedby><cites>FETCH-LOGICAL-c372t-5aaa35b0435e5ef0f79df6259107cc24d7791ac2eb9e1ef92523c095c6dfe3da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24218041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loukovaara, Sirpa</creatorcontrib><creatorcontrib>Lehti, Kaisa</creatorcontrib><creatorcontrib>Robciuc, Alexandra</creatorcontrib><creatorcontrib>Pessi, Timo</creatorcontrib><creatorcontrib>Holopainen, Juha M.</creatorcontrib><creatorcontrib>Koli, Katri</creatorcontrib><creatorcontrib>Immonen, Ilkka</creatorcontrib><creatorcontrib>Keski-Oja, Jorma</creatorcontrib><title>Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Purpose
To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker.
Methods
Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker (control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography.
Results
The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all
p
< 0.01), whereas concentration of VEGF was lower (
p
= 0.01) in eyes with RRD relative to controls. Logistic regression analysis identified Ang-2 concentration as a novel marker of RRD (
p
= 0.0001, OR 48.7). Ang-1, EPO, and total TGF-β1 levels were not significantly different between the groups. However, TGF-β1 and MMP-2 were increased in eyes with total RRD compared to those with local RRD (
p
≤ 0.05). In eyes with PVR, no differences were observed in any studied marker as compared with non-PVR eyes.
Conclusions
Current results reveal Ang-2 as a key factor upregulated in RRD. It may co-operate with fibrosis-associated factors and contribute to vascular complications such as breakdown of blood–eye barrier and PVR development.</description><subject>Aged</subject><subject>Angiopoietin-1 - metabolism</subject><subject>Angiopoietin-2 - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Erythropoietin - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Ophthalmology</subject><subject>Prospective Studies</subject><subject>Retinal Detachment - diagnosis</subject><subject>Retinal Detachment - metabolism</subject><subject>Retinal Disorders</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vitreous Body - metabolism</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp1kM1KAzEYRYMoWqsP4EYG3LiJfkkmprMU8adQcKPQlSHNfNOmzE9N0op9elNaRQRXIeTcm8sh5IzBFQNQ1wEgZ4oCE5RLGND1HumxXEiqgI_3SQ8UZ3Qg-PiIHIcwh4QLyQ7JEc85G6Rsj7wNW-vRBCwz10ZvVi6ma52Zduq6RecwupbyrMYV1iEzIXTWmZjoDxdnmZ_htDGxm2LbLUPmN3QKlxiNnTXYxhNyUJk64Onu7JPXh_uXuyc6en4c3t2OqBWKRyqNMUJONvNQYgWVKsrqhsuCgbKW56VSBTOW46RAhlXBJRcWCmlvygpFaUSfXG57F757X2KIunHBYl2bFtMyzaQAwUHkKqEXf9B5t_Rp9o5ikBcsUWxLWd-F4LHSC-8a4z81A72Rr7fydZKvN_L1OmXOd83LSYPlT-LbdgL4FgjpqZ2i__X1v61fw-qRVQ</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Loukovaara, Sirpa</creator><creator>Lehti, Kaisa</creator><creator>Robciuc, Alexandra</creator><creator>Pessi, Timo</creator><creator>Holopainen, Juha M.</creator><creator>Koli, Katri</creator><creator>Immonen, Ilkka</creator><creator>Keski-Oja, Jorma</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment</title><author>Loukovaara, Sirpa ; Lehti, Kaisa ; Robciuc, Alexandra ; Pessi, Timo ; Holopainen, Juha M. ; Koli, Katri ; Immonen, Ilkka ; Keski-Oja, Jorma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-5aaa35b0435e5ef0f79df6259107cc24d7791ac2eb9e1ef92523c095c6dfe3da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Angiopoietin-1 - metabolism</topic><topic>Angiopoietin-2 - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Erythropoietin - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Ophthalmology</topic><topic>Prospective Studies</topic><topic>Retinal Detachment - diagnosis</topic><topic>Retinal Detachment - metabolism</topic><topic>Retinal Disorders</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vitreous Body - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loukovaara, Sirpa</creatorcontrib><creatorcontrib>Lehti, Kaisa</creatorcontrib><creatorcontrib>Robciuc, Alexandra</creatorcontrib><creatorcontrib>Pessi, Timo</creatorcontrib><creatorcontrib>Holopainen, Juha M.</creatorcontrib><creatorcontrib>Koli, Katri</creatorcontrib><creatorcontrib>Immonen, Ilkka</creatorcontrib><creatorcontrib>Keski-Oja, Jorma</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loukovaara, Sirpa</au><au>Lehti, Kaisa</au><au>Robciuc, Alexandra</au><au>Pessi, Timo</au><au>Holopainen, Juha M.</au><au>Koli, Katri</au><au>Immonen, Ilkka</au><au>Keski-Oja, Jorma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>252</volume><issue>6</issue><spage>881</spage><epage>888</epage><pages>881-888</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Purpose
To explore factors related to pathogenesis of rhegmatogenous retinal detachment (RRD) and development of proliferative vitreoretinopathy (PVR), vitreous levels of angiopoietin-1 and −2 (Ang-1 and −2), previously undefined in RRD, transforming growth factor-(TGF) β1, vascular endothelial growth factor (VEGF), erythropoietin (EPO) and proteolytic mediators of extracellular matrix remodelling (MMP-2 and −9) were compared in eyes with RRD and eyes with idiopathic macular hole or pucker.
Methods
Vitreous samples were collected from 117 eyes with RRD (study group) and 40 eyes with macular hole or pucker (control group). Growth factors were measured by ELISA and matrix metalloproteinases (MMPs) by gelatin zymography.
Results
The mean vitreous concentrations of Ang-2, MMP-2, and MMP-9 were higher (all
p
< 0.01), whereas concentration of VEGF was lower (
p
= 0.01) in eyes with RRD relative to controls. Logistic regression analysis identified Ang-2 concentration as a novel marker of RRD (
p
= 0.0001, OR 48.7). Ang-1, EPO, and total TGF-β1 levels were not significantly different between the groups. However, TGF-β1 and MMP-2 were increased in eyes with total RRD compared to those with local RRD (
p
≤ 0.05). In eyes with PVR, no differences were observed in any studied marker as compared with non-PVR eyes.
Conclusions
Current results reveal Ang-2 as a key factor upregulated in RRD. It may co-operate with fibrosis-associated factors and contribute to vascular complications such as breakdown of blood–eye barrier and PVR development.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>24218041</pmid><doi>10.1007/s00417-013-2508-z</doi><tpages>8</tpages></addata></record> |
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| issn | 0721-832X 1435-702X |
| language | eng |
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| subjects | Aged Angiopoietin-1 - metabolism Angiopoietin-2 - metabolism Biomarkers - metabolism Enzyme-Linked Immunosorbent Assay Erythropoietin - metabolism Female Humans Male Matrix Metalloproteinases - metabolism Medicine Medicine & Public Health Middle Aged Ophthalmology Prospective Studies Retinal Detachment - diagnosis Retinal Detachment - metabolism Retinal Disorders Transforming Growth Factor beta1 - metabolism Vascular Endothelial Growth Factor A - metabolism Vitreous Body - metabolism |
| title | Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment |
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