Evaluation of a Multiple-Variable Thin-Layer and Reversed-Phase Thin-Layer Chromatographic Scheme for the Identification of Basic and Neutral Drugs in an Emergency Toxicology Setting

An analytical scheme composed of one normal-phase thin-layer chromatographic (TLC) method, one reversed-phase thin-layer chromatographic (RPTLC) method, and sequential analyte detection through four stages of color reactions is described. Eighty-one basic or neutral drugs were analyzed with this sch...

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Bibliographic Details
Published in:Journal of analytical toxicology 1989-01, Vol.13 (1), p.31-36
Main Authors: Harper, J.D., Martel, Patricia A., O'Donnell, C. Michael
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography, Thin Layer - methods
Color
Drugs
Emergencies
Gas chromatography
General pharmacology
Isomers
Medical sciences
Pharmaceutical Preparations - analysis
Pharmacology. Drug treatments
Poisoning - diagnosis
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Summary:An analytical scheme composed of one normal-phase thin-layer chromatographic (TLC) method, one reversed-phase thin-layer chromatographic (RPTLC) method, and sequential analyte detection through four stages of color reactions is described. Eighty-one basic or neutral drugs were analyzed with this scheme and seventy-four were uniquely characterized with 95% confidence. Six of the remaining seven formed three unresolved pairs. The scheme was evaluated by mean list length analysis and shown to offer analyte resolution similar to that of a scheme of TLC and gas chromatography (GC) with nonspecific detection. Nine out of ten unknowns from the field of eighty-one drugs were uniquely identified with 95% confidence by the TLC/RPTLC scheme. The tenth unknown was not completely resolved from its isomer, but was statistically the more probable candidate.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/13.1.31