Cordyceps sinensis polysaccharide CPS-2 protects human mesangial cells from PDGF-BB-induced proliferation through the PDGF/ERK and TGF- beta 1/Smad pathways

CPS-2, a Cordyceps sinensis polysaccharide, has been demonstrated to have significant therapeutic activity against chronic renal failure. However, little is known about the underlying molecular mechanism. In this study, we found that CPS-2 could inhibit PDGF-BB-induced human mesangial cells (HMCs) p...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2014-02, Vol.382 (2), p.979-988
Main Authors: Wang, Ying, Liu, Dan, Zhao, Huan, Jiang, Huixing, Luo, Chen, Wang, Min, Yin, Hongping
Format: Article
Language:English
Subjects:
Activation
Cellular
Endocrinology
Failure
Human
Pathways
Polysaccharides
Online Access:Get full text
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Summary:CPS-2, a Cordyceps sinensis polysaccharide, has been demonstrated to have significant therapeutic activity against chronic renal failure. However, little is known about the underlying molecular mechanism. In this study, we found that CPS-2 could inhibit PDGF-BB-induced human mesangial cells (HMCs) proliferation in a dose-dependent manner. In addition, CPS-2 notably suppressed the expression of alpha -SMA, PDGF receptor-beta (PDGFR beta ), TGF- beta 1, and Smad 3 in PDGF-BB-treated HMCs. Furthermore, PDGF-BB-stimulated ERK activation was significantly inhibited by CPS-2, and this inhibitory effect was synergistically potentiated by U0126. CPS-2 could prevent the PDGFR beta promoter activity induced by PDGF-BB, and return expression of PDGFR beta , TGF- beta 1, and TGF beta RI to normal levels while cells were under PDGFR beta and ERK silencing conditions and transfected with DN-ERK. Taken together, these findings demonstrated that CPS-2 reduces PDGF-BB-induced cell proliferation through the PDGF/ERK and TGF- beta 1/Smad pathways, and it may have bi-directional regulatory effects on the PDGF/ERK cellular signaling pathway.
ISSN:0303-7207
DOI:10.1016/j.mce.2013.11.018