maize transposable element Ac excises in progeny of transformed tobacco

To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and their selfed progeny. Ac excised in the majority of primary regenerants and continued to...

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Bibliographic Details
Published in:Plant molecular biology 1989-07, Vol.13 (1), p.109-118
Main Authors: Taylor, B.H, Finnegan, E.J, Dennis, E.S, Peacock, W.J
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Biological and medical sciences
Blotting, Southern
cloning
DNA - genetics
DNA - isolation & purification
DNA methylation
DNA Transposable Elements
Fundamental and applied biological sciences. Psychology
gene tagging
genetic markers
genetic transformation
Genetic Vectors
genetically modified organisms
Genic rearrangement. Recombination. Transposable element
insertional mutagenesis
Molecular and cellular biology
Molecular genetics
mutagenesis
Nicotiana - genetics
Nicotiana tabacum
Plants, Genetically Modified
Plants, Toxic
Restriction Mapping
transcription (genetics)
Transcription, Genetic
Transformation, Genetic
transposition
transposons
Zea mays
Zea mays - genetics
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Summary:To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and their selfed progeny. Ac excised in the majority of primary regenerants and continued to excise in the first-generation progeny plants. In one primary regenerant Ac was transcribed but did not excise. Fourteen of eighteen kanamycin-resistant progeny from this plant showed Ac excision, suggesting that excision of Ac may have been activated during meiosis or in embryo development. This finding, together with the more general observation of continued Ac mobility in the progeny of transformed plants in which Ac had excised, suggests that Ac will be useful for gene tagging.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00027339