Characterization of the Escherichia coli chlC regulatory region in a cloned chlC‐lac gene fusion

Since the immediate product of the chlC gene has no known enzymatically assayable activity (in the absence of required cofactors), the authors have used a chlc-lacZ gene fusion to study the specific regulation of the chlC gene and have observed that it is under the same control as that which regulat...

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Bibliographic Details
Published in:FEMS microbiology letters 1981-01, Vol.12 (2), p.125-129
Main Authors: Fimmel, Anthony L., Haddock, Bruce A.
Format: Article
Language:English
Subjects:
Escherichia coli
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Summary:Since the immediate product of the chlC gene has no known enzymatically assayable activity (in the absence of required cofactors), the authors have used a chlc-lacZ gene fusion to study the specific regulation of the chlC gene and have observed that it is under the same control as that which regulates the synthesis of nitrate reductase. To facilitate studies on the nature of anaerobic regulation of gene expression at the DNA nucleotide level the authors have cloned the chlC-lacZ gene fusion initially into phage and then into plasmid pBR322.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1981.tb07625.x