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A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol
A uniparental mutant of Chlamydomonas resistant to chloramphenicol was selected following treatment of wild-type cells with 5-fluorodeoxyuridine. Under heterotrophic conditions, growth and chloroplast protein synthesis of this mutant (CAP1) are resistant to chloramphenicol. Under phototrophic condit...
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Published in: | Current genetics 1981-07, Vol.3 (3), p.251-253 |
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container_end_page | 253 |
container_issue | 3 |
container_start_page | 251 |
container_title | Current genetics |
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creator | Bennoun, P Delepelaire, P Delosme, M |
description | A uniparental mutant of Chlamydomonas resistant to chloramphenicol was selected following treatment of wild-type cells with 5-fluorodeoxyuridine. Under heterotrophic conditions, growth and chloroplast protein synthesis of this mutant (CAP1) are resistant to chloramphenicol. Under phototrophic conditions, CAP1 is sensitive to chloramphenicol. In addition CAP1 displays thermosensitivity when grown phototrophically in the absence of antibiotics: at the restrictive temperature, a specific reduction of those thylakoid membrane polypeptides which are synthesized inside the chloroplast is observed. Alternative explanations for the pleiotropic phenotype of CAP1 are discussed. |
doi_str_mv | 10.1007/BF00429829 |
format | article |
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Inst. de Biologie Physico-Chimique</creatorcontrib><description>A uniparental mutant of Chlamydomonas resistant to chloramphenicol was selected following treatment of wild-type cells with 5-fluorodeoxyuridine. Under heterotrophic conditions, growth and chloroplast protein synthesis of this mutant (CAP1) are resistant to chloramphenicol. Under phototrophic conditions, CAP1 is sensitive to chloramphenicol. In addition CAP1 displays thermosensitivity when grown phototrophically in the absence of antibiotics: at the restrictive temperature, a specific reduction of those thylakoid membrane polypeptides which are synthesized inside the chloroplast is observed. 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Inst. de Biologie Physico-Chimique</creatorcontrib><title>A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>A uniparental mutant of Chlamydomonas resistant to chloramphenicol was selected following treatment of wild-type cells with 5-fluorodeoxyuridine. Under heterotrophic conditions, growth and chloroplast protein synthesis of this mutant (CAP1) are resistant to chloramphenicol. Under phototrophic conditions, CAP1 is sensitive to chloramphenicol. In addition CAP1 displays thermosensitivity when grown phototrophically in the absence of antibiotics: at the restrictive temperature, a specific reduction of those thylakoid membrane polypeptides which are synthesized inside the chloroplast is observed. 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Inst. de Biologie Physico-Chimique</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bennoun, P</au><au>Delepelaire, P</au><au>Delosme, M</au><aucorp>Paris-6 Univ., 75 (France). Inst. de Biologie Physico-Chimique</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1981-07</date><risdate>1981</risdate><volume>3</volume><issue>3</issue><spage>251</spage><epage>253</epage><pages>251-253</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>A uniparental mutant of Chlamydomonas resistant to chloramphenicol was selected following treatment of wild-type cells with 5-fluorodeoxyuridine. Under heterotrophic conditions, growth and chloroplast protein synthesis of this mutant (CAP1) are resistant to chloramphenicol. Under phototrophic conditions, CAP1 is sensitive to chloramphenicol. In addition CAP1 displays thermosensitivity when grown phototrophically in the absence of antibiotics: at the restrictive temperature, a specific reduction of those thylakoid membrane polypeptides which are synthesized inside the chloroplast is observed. Alternative explanations for the pleiotropic phenotype of CAP1 are discussed.</abstract><cop>United States</cop><pmid>24190139</pmid><doi>10.1007/BF00429829</doi><tpages>3</tpages></addata></record> |
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language | eng |
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source | Springer LINK Archives |
subjects | Chlamydomonas reinhardtii |
title | A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol |
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