Suramin inhibits cell growth and glycolytic activity and triggers differentiation of human colic adenocarcinoma cell clone HT29-D4

Suramin, a drug used in the treatment of trypanosomiasis and onchocerciasis inhibits growth factor-induced mitogenesis. In the present report, we show that suramin inhibits the growth of human colic adenocarcinoma cells HT29-D4 and rapidly induces their differentiation into enterocyte-like cells. As...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-06, Vol.264 (17), p.10282-10286
Main Authors: Fantini, J, Rognoni, J B, Roccabianca, M, Pommier, G, Marvaldi, J
Format: Article
Language:English
Subjects:
Adenocarcinoma
Alkaline Phosphatase - metabolism
Biological and medical sciences
Cell Differentiation - drug effects
Cell differentiation, maturation, development, hematopoiesis
Cell Division - drug effects
Cell Line
Cell physiology
colon
Colonic Neoplasms
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Glycolysis - drug effects
Humans
Kinetics
Microscopy, Electron
Molecular and cellular biology
Sucrase-Isomaltase Complex - metabolism
Suramin - pharmacology
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - ultrastructure
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Summary:Suramin, a drug used in the treatment of trypanosomiasis and onchocerciasis inhibits growth factor-induced mitogenesis. In the present report, we show that suramin inhibits the growth of human colic adenocarcinoma cells HT29-D4 and rapidly induces their differentiation into enterocyte-like cells. As soon as 6 days after the addition of suramin (100 µg/ml) in the culture medium, the cells form a polarized monolayer of regular columnar cells with occluding junctions delimiting two distinct membrane domains (apical and basolateral) and an apical brush-border expressing alkaline phosphatase and sucrase-isomaltase. The process of differentiation is fully reversible when the drug is removed from the culture medium. We also show that suramin inhibits both glucose consumption and lactate production so that the glycolytic activity of the treated cells is lowered by 42%. This observation would shed some light on the complex mechanisms involved during the induction of HT29 cell differentiation when glucose is removed from the culture medium.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)81797-5