Absence of induction of enhanced reactivation of herpes simplex virus in cells from Xeroderma pigmentosum patients without skin cancer

The time course of appearance of enhanced reactivation (ER) and enhanced mutagenesis (EM) of herpes simplex virus type 1 were studied in UV-irradiated stationary cultures of xeroderma pigmentosum (XP) fibroblasts. In some of the XP cells EM followed similar kinetics of appearance as ER. Maximal acti...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1988-11, Vol.48 (21), p.6054-6057
Main Authors: ABRAHAMS, P. J, VAN DER KLEIJ, A. A. M, SCHOUTEN, R, VAN DER EB, A. J
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Cells, Cultured
Child
Child, Preschool
Dermatology
DNA Repair
Dose-Response Relationship, Radiation
Female
Hereditary diseases of the skin. Congenital diseases of the skin. Haemangioma of the skin, of mucosae and of soft tissue
Humans
Infant
Male
Medical sciences
Mutation
Simplexvirus - growth & development
Ultraviolet Rays
Virus Activation
Xeroderma Pigmentosum - microbiology
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Summary:The time course of appearance of enhanced reactivation (ER) and enhanced mutagenesis (EM) of herpes simplex virus type 1 were studied in UV-irradiated stationary cultures of xeroderma pigmentosum (XP) fibroblasts. In some of the XP cells EM followed similar kinetics of appearance as ER. Maximal activities occurred when infection was delayed 1 or 2 days after cell treatment. However, in certain XP cells only induction of the EM response was observed, whereas ER was absent. Interestingly, the latter XP cells had been obtained from patients who had not yet developed skin cancer at the time they were described in the literature, whereas the former XP patients had already developed skin tumors. This suggests that the ER response may somehow be involved in the process of oncogenic transformation. Dose-response studies of ER in XP cells from tumor-bearing patients showed that ER is maximally induced with a UV dose of 40 Jm-2 given to the virus. Normal levels of ER were observed in 14 different normal human skin fibroblasts, indicating that the ER- phenotype does not occur in normal cells or at least more rarely than in XP cells.
ISSN:0008-5472
1538-7445