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Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur
Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, live...
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Published in: | Journal of agricultural and food chemistry 2014-06, Vol.62 (22), p.5011-5019 |
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container_title | Journal of agricultural and food chemistry |
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creator | Feng, Shixia Chiesa, Oscar A Kijak, Philip Chattopadhaya, Chaitali Lancaster, Vicki Smith, Elizabeth A Girard, Lauren Sklenka, Sara Li, Hui |
description | Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. It was concluded that DCCD is a suitable surrogate marker residue for ceftiofur in bovine kidney. |
doi_str_mv | 10.1021/jf405423e |
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This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. It was concluded that DCCD is a suitable surrogate marker residue for ceftiofur in bovine kidney.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf405423e</identifier><identifier>PMID: 24819974</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>acetonitrile ; Animals ; Anti-Bacterial Agents - analysis ; Anti-Bacterial Agents - metabolism ; cattle ; Cattle - metabolism ; ceftiofur ; Cephalosporins - analysis ; Cephalosporins - metabolism ; Chromatography, Liquid - methods ; cysteine ; Cysteine - analysis ; Cysteine - metabolism ; Disulfides - analysis ; Disulfides - metabolism ; Drug Residues - analysis ; Drug Residues - metabolism ; hexane ; Kidney - chemistry ; Kidney - metabolism ; kidneys ; liquid chromatography ; liver ; Liver - chemistry ; Liver - metabolism ; metabolites ; muscle tissues ; muscles ; Muscles - chemistry ; Muscles - metabolism ; phosphates ; proteins ; solid phase extraction ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of agricultural and food chemistry, 2014-06, Vol.62 (22), p.5011-5019</ispartof><rights>Copyright © 2014 U.S. Government</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a339t-259269bfc8dd0ce0ff4e050863a55fa9039e9e456b3b99f2330dff6005aec61d3</citedby><cites>FETCH-LOGICAL-a339t-259269bfc8dd0ce0ff4e050863a55fa9039e9e456b3b99f2330dff6005aec61d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24819974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Shixia</creatorcontrib><creatorcontrib>Chiesa, Oscar A</creatorcontrib><creatorcontrib>Kijak, Philip</creatorcontrib><creatorcontrib>Chattopadhaya, Chaitali</creatorcontrib><creatorcontrib>Lancaster, Vicki</creatorcontrib><creatorcontrib>Smith, Elizabeth A</creatorcontrib><creatorcontrib>Girard, Lauren</creatorcontrib><creatorcontrib>Sklenka, Sara</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><title>Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. It was concluded that DCCD is a suitable surrogate marker residue for ceftiofur in bovine kidney.</description><subject>acetonitrile</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>cattle</subject><subject>Cattle - metabolism</subject><subject>ceftiofur</subject><subject>Cephalosporins - analysis</subject><subject>Cephalosporins - metabolism</subject><subject>Chromatography, Liquid - methods</subject><subject>cysteine</subject><subject>Cysteine - analysis</subject><subject>Cysteine - metabolism</subject><subject>Disulfides - analysis</subject><subject>Disulfides - metabolism</subject><subject>Drug Residues - analysis</subject><subject>Drug Residues - metabolism</subject><subject>hexane</subject><subject>Kidney - chemistry</subject><subject>Kidney - metabolism</subject><subject>kidneys</subject><subject>liquid chromatography</subject><subject>liver</subject><subject>Liver - chemistry</subject><subject>Liver - metabolism</subject><subject>metabolites</subject><subject>muscle tissues</subject><subject>muscles</subject><subject>Muscles - chemistry</subject><subject>Muscles - metabolism</subject><subject>phosphates</subject><subject>proteins</subject><subject>solid phase extraction</subject><subject>tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNptkc-O0zAQxi0EYruFAy8AviAth8A4jtP4CFn-Sa2QaHuOpsm465LEXTtByo134IV4Fp4Er7qUC6cZzffTN2N_jD0T8FpAKt4cTAYqSyU9YDOhUkiUEMVDNoMoJoXKxQW7DOEAAIVawGN2kWaF0HqRzdivaxrId7bHwbqeO8NLMrE1o-crGnDnWjsQv6YQJ25q67NaTmEg20fNhrE1tiFue_7Ofb-bbWwIIwW-Dbbf86W9HW3DyxvvOhzc3uPxZvr94-cG-4Y6vsIQ-PpI9RB1GvzEMXDk69F7t8e4fYX-G3n-lYJtRuLG-X9XPmGPDLaBnt7XOdt-eL8pPyXLLx8_l2-XCUqphyRVOs31ztRF00BNYExGoKDIJSplUIPUpClT-U7utDaplNAYkwMopDoXjZyzq5Pv0bvb-LSh6myoqW2xJzeGSiiZagU6Os7ZqxNaexeCJ1Mdve3QT5WA6i6w6hxYZJ_f2467jpoz-TehCLw4AQZdhXtvQ7VdpyDiaULoRaEj8fJEYB2qgxt9H__hP6v-ALwnq9Q</recordid><startdate>20140604</startdate><enddate>20140604</enddate><creator>Feng, Shixia</creator><creator>Chiesa, Oscar A</creator><creator>Kijak, Philip</creator><creator>Chattopadhaya, Chaitali</creator><creator>Lancaster, Vicki</creator><creator>Smith, Elizabeth A</creator><creator>Girard, Lauren</creator><creator>Sklenka, Sara</creator><creator>Li, Hui</creator><general>American Chemical Society</general><general>American Chemical Society, Books and Journals Division</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140604</creationdate><title>Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur</title><author>Feng, Shixia ; Chiesa, Oscar A ; Kijak, Philip ; Chattopadhaya, Chaitali ; Lancaster, Vicki ; Smith, Elizabeth A ; Girard, Lauren ; Sklenka, Sara ; Li, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a339t-259269bfc8dd0ce0ff4e050863a55fa9039e9e456b3b99f2330dff6005aec61d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>acetonitrile</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>cattle</topic><topic>Cattle - metabolism</topic><topic>ceftiofur</topic><topic>Cephalosporins - analysis</topic><topic>Cephalosporins - metabolism</topic><topic>Chromatography, Liquid - methods</topic><topic>cysteine</topic><topic>Cysteine - analysis</topic><topic>Cysteine - metabolism</topic><topic>Disulfides - analysis</topic><topic>Disulfides - metabolism</topic><topic>Drug Residues - analysis</topic><topic>Drug Residues - metabolism</topic><topic>hexane</topic><topic>Kidney - chemistry</topic><topic>Kidney - metabolism</topic><topic>kidneys</topic><topic>liquid chromatography</topic><topic>liver</topic><topic>Liver - chemistry</topic><topic>Liver - metabolism</topic><topic>metabolites</topic><topic>muscle tissues</topic><topic>muscles</topic><topic>Muscles - chemistry</topic><topic>Muscles - metabolism</topic><topic>phosphates</topic><topic>proteins</topic><topic>solid phase extraction</topic><topic>tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Shixia</creatorcontrib><creatorcontrib>Chiesa, Oscar A</creatorcontrib><creatorcontrib>Kijak, Philip</creatorcontrib><creatorcontrib>Chattopadhaya, Chaitali</creatorcontrib><creatorcontrib>Lancaster, Vicki</creatorcontrib><creatorcontrib>Smith, Elizabeth A</creatorcontrib><creatorcontrib>Girard, Lauren</creatorcontrib><creatorcontrib>Sklenka, Sara</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Shixia</au><au>Chiesa, Oscar A</au><au>Kijak, Philip</au><au>Chattopadhaya, Chaitali</au><au>Lancaster, Vicki</au><au>Smith, Elizabeth A</au><au>Girard, Lauren</au><au>Sklenka, Sara</au><au>Li, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2014-06-04</date><risdate>2014</risdate><volume>62</volume><issue>22</issue><spage>5011</spage><epage>5019</epage><pages>5011-5019</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. It was concluded that DCCD is a suitable surrogate marker residue for ceftiofur in bovine kidney.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24819974</pmid><doi>10.1021/jf405423e</doi><tpages>9</tpages></addata></record> |
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subjects | acetonitrile Animals Anti-Bacterial Agents - analysis Anti-Bacterial Agents - metabolism cattle Cattle - metabolism ceftiofur Cephalosporins - analysis Cephalosporins - metabolism Chromatography, Liquid - methods cysteine Cysteine - analysis Cysteine - metabolism Disulfides - analysis Disulfides - metabolism Drug Residues - analysis Drug Residues - metabolism hexane Kidney - chemistry Kidney - metabolism kidneys liquid chromatography liver Liver - chemistry Liver - metabolism metabolites muscle tissues muscles Muscles - chemistry Muscles - metabolism phosphates proteins solid phase extraction tandem mass spectrometry Tandem Mass Spectrometry - methods |
title | Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur |
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