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Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur

Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, live...

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Published in:Journal of agricultural and food chemistry 2014-06, Vol.62 (22), p.5011-5019
Main Authors: Feng, Shixia, Chiesa, Oscar A, Kijak, Philip, Chattopadhaya, Chaitali, Lancaster, Vicki, Smith, Elizabeth A, Girard, Lauren, Sklenka, Sara, Li, Hui
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cited_by cdi_FETCH-LOGICAL-a339t-259269bfc8dd0ce0ff4e050863a55fa9039e9e456b3b99f2330dff6005aec61d3
cites cdi_FETCH-LOGICAL-a339t-259269bfc8dd0ce0ff4e050863a55fa9039e9e456b3b99f2330dff6005aec61d3
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container_issue 22
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container_title Journal of agricultural and food chemistry
container_volume 62
creator Feng, Shixia
Chiesa, Oscar A
Kijak, Philip
Chattopadhaya, Chaitali
Lancaster, Vicki
Smith, Elizabeth A
Girard, Lauren
Sklenka, Sara
Li, Hui
description Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. It was concluded that DCCD is a suitable surrogate marker residue for ceftiofur in bovine kidney.
doi_str_mv 10.1021/jf405423e
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This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. 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Agric. Food Chem</addtitle><description>Ceftiofur is a widely used cephalosporin β-lactam antibiotic with frequently reported residue violations. This paper reports a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for determining a ceftiofur metabolite, desfuroylceftiofur cysteine disulfide (DCCD), in bovine kidney, liver, and muscle tissues. Incurred tissue samples were obtained from dosed animals and analyzed to evaluate the utility of the method. For kidney, the target tissue, the method utilized a simple extraction with phosphate buffer followed by solid phase extraction (SPE) cleanup. For liver and muscle, acetonitrile and hexane were used to remove most proteins and fat from the initial buffer extract before the SPE cleanup. Method accuracy was between 97 and 107%, and the coefficient of variation was between 3.4 and 11.0% for all three types of tissues. The relationship between the new and regulatory methods for bovine kidney was established. 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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects acetonitrile
Animals
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - metabolism
cattle
Cattle - metabolism
ceftiofur
Cephalosporins - analysis
Cephalosporins - metabolism
Chromatography, Liquid - methods
cysteine
Cysteine - analysis
Cysteine - metabolism
Disulfides - analysis
Disulfides - metabolism
Drug Residues - analysis
Drug Residues - metabolism
hexane
Kidney - chemistry
Kidney - metabolism
kidneys
liquid chromatography
liver
Liver - chemistry
Liver - metabolism
metabolites
muscle tissues
muscles
Muscles - chemistry
Muscles - metabolism
phosphates
proteins
solid phase extraction
tandem mass spectrometry
Tandem Mass Spectrometry - methods
title Determination of Ceftiofur Metabolite Desfuroylceftiofur Cysteine Disulfide in Bovine Tissues Using Liquid Chromatography–Tandem Mass Spectrometry as a Surrogate Marker Residue for Ceftiofur
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