Cytolytic T Lymphocyte Activity and Delayed-Type Hypersensitivity in Rejecting- and Tolerant-Lung Allografts

The authors examined effector mechanisms of allograft destruction in a canine single-lung transplantation model. Intragraft effector cells were obtained from the transplanted lung by bronchoalveolar lavage (BAL). Cells were also studied from BAL fluid of the normal lung and peripheral blood. Intragr...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1988, Vol.532 (1), p.456-458
Main Authors: NORIN, ALLEN J., KAMHOLZ, STEPHAN L., PINSKER, KENNETH L., VEITH, FRANK J.
Format: Article
Language:English
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Summary:The authors examined effector mechanisms of allograft destruction in a canine single-lung transplantation model. Intragraft effector cells were obtained from the transplanted lung by bronchoalveolar lavage (BAL). Cells were also studied from BAL fluid of the normal lung and peripheral blood. Intragraft CTL activity was quantified in standard super(51)Cr-release assays against donor lymphocytes. To measure DTH reactions they used a lectin (concanavalin A, (Con A)-dependent cell mediated-cytotoxicity assay (CDCMC), which is known to detect CTL and interleukin-2 (I1-2)-activated killer cells, IAK (also known as lymphokine-activated killer cells, LAK), but not natural killer cells (NK).
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1988.tb36374.x