Low Molecular Weight RNAs Transcribed in vitro by RNA Polymerase III from Alu-Type Dispersed Repeats in Chinese Hamster DNA are also Found in vivo

• Published in: Proceedings of the National Academy of Sciences - PNAS 1981-10, Vol.78 (10), p.6130-6134
• Main Authors: Haynes, Susan R., Jelinek, Warren R.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Cell Line; CHO cells; Cloning, Molecular; Cricetinae; Cricetulus; DNA; DNA - genetics; DNA, Recombinant - metabolism; DNA-Directed RNA Polymerases - metabolism; Family members; Female; Gels; Low molecular weights; Molecular Weight; Nucleic Acid Hybridization; Nucleotide sequences; Nucleotides; Oligonucleotides; Oligoribonucleotides - analysis; Ovary; Product labeling; Ribonuclease T1; RNA; RNA Polymerase III - metabolism; Transcription, Genetic
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.78.10.6130

An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA [Haynes, S. R., Toomey, T. P., Leinwand, L. & Jelinek, W. R. (1981) Mol. Cell. Biol. 1, 573-583] serves as a template for cell-free transcription of discrete low molecular weight RNAs by RNA polymerase III [RNA nucleotidyltransferase (DNA-directed), EC 2.7.7.6]. A class of analogous RNAs has been isolated from growing Chinese hamster cells by hybridization of total low molecular weight nuclear RNAs to the cloned DNA fragment from which cell-free transcription occurs. Two-dimensional analysis of RNase digestion products of these RNAs suggests that they are transcribed from multiple members of the Alu-type dispersed repeat family.