Oral dosing in adult zebrafish: Proof-of-concept using pharmacokinetics and pharmacological evaluation of carbamazepine

We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated...

Full description

Saved in:
Bibliographic Details
Published in:Pharmacological reports 2014-02, Vol.66 (1), p.179-183
Main Authors: Kulkarni, Pushkar, Chaudhari, Girish Hari, Sripuram, Vijaykumar, Banote, Rakesh Kumar, Kirla, Krishna Tulasi, Sultana, Razia, Rao, Pallavi, Oruganti, Srinivas, Chatti, Kiranam
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Anti-Anxiety Agents - pharmacology
Blood-Brain Barrier
Carbamazepine - pharmacokinetics
Carbamazepine - pharmacology
Drug delivery
Drug discovery
Drug Safety and Pharmacovigilance
Male
Oral administration
Pharmacokinetics
Pharmacotherapy
Pharmacy
Short Communication
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe a method for obtaining pharmacokinetics (PK) and pharmacology data from adult zebrafish in terms of mg/kg using a novel method of oral administration. Using carbamazepine (CBZ) as a test drug, we employed dried blood spot (DBS) cards to enable drug quantification for PK; and we evaluated the pharmacological anxiolytic effect using novel tank test. The PK study confirmed the presence of CBZ in both blood and brain and the behavioural study showed dose dependent anxiolytic effect. The reproducibility of oral dosing was confirmed by the fact that the results obtained in both the experiments had negligible errors. This report enables a novel approach for optimizing the utility of zebrafish in drug discovery and drug delivery research.
ISSN:1734-1140
2299-5684
DOI:10.1016/j.pharep.2013.06.012