Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation

Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment....

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Published in:Transfusion and apheresis science 2014-06, Vol.50 (3), p.443-450
Main Authors: D’Rozario, James, Parisotto, Robin, Stapleton, Jennifer, Gidley, Alison, Owen, David
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Amyloidosis - therapy
Antigens, CD34
Autografts
Autologous transplantation
CD34+ cell count
Cryopreservation
Female
Graft Survival
Health technology assessment
Hematology, Oncology and Palliative Medicine
Hematopoietic Stem Cells
Humans
Male
Middle Aged
Multiple Sclerosis - therapy
Neoplasms - therapy
Neutrophil engraftment
Neutrophils
Peripheral Blood Stem Cell Transplantation
Platelet engraftment
Retrospective Studies
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container_end_page 450
container_issue 3
container_start_page 443
container_title Transfusion and apheresis science
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creator D’Rozario, James
Parisotto, Robin
Stapleton, Jennifer
Gidley, Alison
Owen, David
description Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment. Viable CD34+ cell numbers are enumerated in most laboratories using the ISHAGE single platform flow cytometric method which utilizes monoclonal antibodies to CD45, CD34 and 7 amino actinomycin D (7AAD) dye exclusion. Methods One hundred and six consecutive autologous transplantation procedures underwent viable CD34+ cell enumeration at the time of harvesting and post thaw prior to re-infusion. Neutrophil and platelet engraftment and markers of haematopoietic support were analyzed. Results Mean pre-cryopreservation viable CD34+ numbers were 4.882 × 106 /kg. Mean post thaw viable CD34+ numbers were 3.234 × 106 /kg. Mean loss of viable CD34+ cells with processing and cryo-preservation was 1.648 × 106 /kg (33%). For neutrophil engraftment, there was no significant difference between high (⩾3.0 × 106 /kg) and low (
doi_str_mv 10.1016/j.transci.2014.02.021
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A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment. Viable CD34+ cell numbers are enumerated in most laboratories using the ISHAGE single platform flow cytometric method which utilizes monoclonal antibodies to CD45, CD34 and 7 amino actinomycin D (7AAD) dye exclusion. Methods One hundred and six consecutive autologous transplantation procedures underwent viable CD34+ cell enumeration at the time of harvesting and post thaw prior to re-infusion. Neutrophil and platelet engraftment and markers of haematopoietic support were analyzed. Results Mean pre-cryopreservation viable CD34+ numbers were 4.882 × 106 /kg. Mean post thaw viable CD34+ numbers were 3.234 × 106 /kg. Mean loss of viable CD34+ cells with processing and cryo-preservation was 1.648 × 106 /kg (33%). For neutrophil engraftment, there was no significant difference between high (⩾3.0 × 106 /kg) and low (&lt;1.5 × 106 /kg) post thaw viable CD34+ cell counts ( p = 0.545). For platelet engraftment, there was however a significant difference observed between the high and low pre infusion viable CD34+ groups ( p &lt; 0.001). Additionally, significant differences were seen between the post thaw viable CD34+ cell count and the associated length of hospital admission, days of use of G-CSF post transplantation, use of antibiotics in the post transplantation period and transfusion support in the post transplantation period. Conclusion A significant loss of viable CD34+ cells occurs during processing, cryopreservation and thawing. Low numbers of viable CD34+ cells infused post thaw will still result in adequate neutrophil engraftment however may delay platelet engraftment. Low viable CD34+ cell numbers have significant effects on admission duration and use of haematopoietic supportive measures with consequent effects on healthcare resources.</description><identifier>ISSN: 1473-0502</identifier><identifier>EISSN: 1878-1683</identifier><identifier>DOI: 10.1016/j.transci.2014.02.021</identifier><identifier>PMID: 24680293</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Amyloidosis - therapy ; Antigens, CD34 ; Autografts ; Autologous transplantation ; CD34+ cell count ; Cryopreservation ; Female ; Graft Survival ; Health technology assessment ; Hematology, Oncology and Palliative Medicine ; Hematopoietic Stem Cells ; Humans ; Male ; Middle Aged ; Multiple Sclerosis - therapy ; Neoplasms - therapy ; Neutrophil engraftment ; Neutrophils ; Peripheral Blood Stem Cell Transplantation ; Platelet engraftment ; Retrospective Studies</subject><ispartof>Transfusion and apheresis science, 2014-06, Vol.50 (3), p.443-450</ispartof><rights>2014</rights><rights>Crown Copyright © 2014. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-6298b13d9f71f8dad6b7460cce704752aed5bc9462ec3519b6893b4f2f612c9c3</citedby><cites>FETCH-LOGICAL-c490t-6298b13d9f71f8dad6b7460cce704752aed5bc9462ec3519b6893b4f2f612c9c3</cites><orcidid>0000-0001-5219-5282 ; 0000-0002-5983-753X ; 0000-0003-4837-5752</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24680293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D’Rozario, James</creatorcontrib><creatorcontrib>Parisotto, Robin</creatorcontrib><creatorcontrib>Stapleton, Jennifer</creatorcontrib><creatorcontrib>Gidley, Alison</creatorcontrib><creatorcontrib>Owen, David</creatorcontrib><title>Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation</title><title>Transfusion and apheresis science</title><addtitle>Transfus Apher Sci</addtitle><description>Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment. Viable CD34+ cell numbers are enumerated in most laboratories using the ISHAGE single platform flow cytometric method which utilizes monoclonal antibodies to CD45, CD34 and 7 amino actinomycin D (7AAD) dye exclusion. Methods One hundred and six consecutive autologous transplantation procedures underwent viable CD34+ cell enumeration at the time of harvesting and post thaw prior to re-infusion. Neutrophil and platelet engraftment and markers of haematopoietic support were analyzed. Results Mean pre-cryopreservation viable CD34+ numbers were 4.882 × 106 /kg. Mean post thaw viable CD34+ numbers were 3.234 × 106 /kg. Mean loss of viable CD34+ cells with processing and cryo-preservation was 1.648 × 106 /kg (33%). For neutrophil engraftment, there was no significant difference between high (⩾3.0 × 106 /kg) and low (&lt;1.5 × 106 /kg) post thaw viable CD34+ cell counts ( p = 0.545). For platelet engraftment, there was however a significant difference observed between the high and low pre infusion viable CD34+ groups ( p &lt; 0.001). Additionally, significant differences were seen between the post thaw viable CD34+ cell count and the associated length of hospital admission, days of use of G-CSF post transplantation, use of antibiotics in the post transplantation period and transfusion support in the post transplantation period. Conclusion A significant loss of viable CD34+ cells occurs during processing, cryopreservation and thawing. Low numbers of viable CD34+ cells infused post thaw will still result in adequate neutrophil engraftment however may delay platelet engraftment. Low viable CD34+ cell numbers have significant effects on admission duration and use of haematopoietic supportive measures with consequent effects on healthcare resources.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Amyloidosis - therapy</subject><subject>Antigens, CD34</subject><subject>Autografts</subject><subject>Autologous transplantation</subject><subject>CD34+ cell count</subject><subject>Cryopreservation</subject><subject>Female</subject><subject>Graft Survival</subject><subject>Health technology assessment</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Hematopoietic Stem Cells</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Multiple Sclerosis - therapy</subject><subject>Neoplasms - therapy</subject><subject>Neutrophil engraftment</subject><subject>Neutrophils</subject><subject>Peripheral Blood Stem Cell Transplantation</subject><subject>Platelet engraftment</subject><subject>Retrospective Studies</subject><issn>1473-0502</issn><issn>1878-1683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFUtuq1DAULaJ4LvoJSh4FT8fcmrYvioxXOKCgPoc03TmTMU1qkirne_xR05nRh_MibEhgr7X2Ze2qekLwhmAiXuw3OSqftN1QTPgG0xLkXnVOurariejY_fLnLatxg-lZdZHSHmPSkl48rM4oFx2mPTuvfn-OgKw3S7LBX6E5pIzyTv1C2zeMP0czRDvvICqHBhfCiFKGCWlwDoFfppLIhYdUQgrNEUarc4goGLRTMKkc5mAhW13AN1GZPIHPpRpSSw4u3IQl3QUepA-TzU75fJB_VD0wyiV4fHovq2_v3n7dfqivP73_uH19XWve41wL2ncDYWNvWmK6UY1iaLnAWkOLedtQBWMz6J4LCpo1pB9E17OBG2oEobrX7LJ6dtSdY_ixQMpysmltSHkorUrSMM6F4JgUaHOE6hhSimDkHO2k4q0kWK7-yL08-SNXfySmJVbe01OJZZhg_Mf6a0gBvDoCoAz600KURQK8LquNoLMcg_1viZd3FLSz3mrlvsMtpH1Yoi9blESmQpBf1iNZb4RwjHGZjv0Bywq8yg</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>D’Rozario, James</creator><creator>Parisotto, Robin</creator><creator>Stapleton, Jennifer</creator><creator>Gidley, Alison</creator><creator>Owen, David</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5219-5282</orcidid><orcidid>https://orcid.org/0000-0002-5983-753X</orcidid><orcidid>https://orcid.org/0000-0003-4837-5752</orcidid></search><sort><creationdate>20140601</creationdate><title>Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation</title><author>D’Rozario, James ; Parisotto, Robin ; Stapleton, Jennifer ; Gidley, Alison ; Owen, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-6298b13d9f71f8dad6b7460cce704752aed5bc9462ec3519b6893b4f2f612c9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Amyloidosis - therapy</topic><topic>Antigens, CD34</topic><topic>Autografts</topic><topic>Autologous transplantation</topic><topic>CD34+ cell count</topic><topic>Cryopreservation</topic><topic>Female</topic><topic>Graft Survival</topic><topic>Health technology assessment</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Hematopoietic Stem Cells</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Multiple Sclerosis - therapy</topic><topic>Neoplasms - therapy</topic><topic>Neutrophil engraftment</topic><topic>Neutrophils</topic><topic>Peripheral Blood Stem Cell Transplantation</topic><topic>Platelet engraftment</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>D’Rozario, James</creatorcontrib><creatorcontrib>Parisotto, Robin</creatorcontrib><creatorcontrib>Stapleton, Jennifer</creatorcontrib><creatorcontrib>Gidley, Alison</creatorcontrib><creatorcontrib>Owen, David</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion and apheresis science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>D’Rozario, James</au><au>Parisotto, Robin</au><au>Stapleton, Jennifer</au><au>Gidley, Alison</au><au>Owen, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation</atitle><jtitle>Transfusion and apheresis science</jtitle><addtitle>Transfus Apher Sci</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>50</volume><issue>3</issue><spage>443</spage><epage>450</epage><pages>443-450</pages><issn>1473-0502</issn><eissn>1878-1683</eissn><abstract>Abstract Introduction By convention, peripheral blood stem cell products for autologous transplantation are evaluated for quality by CD34+ cell dose at the time of harvesting. A CD34+ cell dose in excess of 2.0 × 106 /kg of recipient body weight is considered adequate for haematopoietic engraftment. Viable CD34+ cell numbers are enumerated in most laboratories using the ISHAGE single platform flow cytometric method which utilizes monoclonal antibodies to CD45, CD34 and 7 amino actinomycin D (7AAD) dye exclusion. Methods One hundred and six consecutive autologous transplantation procedures underwent viable CD34+ cell enumeration at the time of harvesting and post thaw prior to re-infusion. Neutrophil and platelet engraftment and markers of haematopoietic support were analyzed. Results Mean pre-cryopreservation viable CD34+ numbers were 4.882 × 106 /kg. Mean post thaw viable CD34+ numbers were 3.234 × 106 /kg. Mean loss of viable CD34+ cells with processing and cryo-preservation was 1.648 × 106 /kg (33%). For neutrophil engraftment, there was no significant difference between high (⩾3.0 × 106 /kg) and low (&lt;1.5 × 106 /kg) post thaw viable CD34+ cell counts ( p = 0.545). For platelet engraftment, there was however a significant difference observed between the high and low pre infusion viable CD34+ groups ( p &lt; 0.001). Additionally, significant differences were seen between the post thaw viable CD34+ cell count and the associated length of hospital admission, days of use of G-CSF post transplantation, use of antibiotics in the post transplantation period and transfusion support in the post transplantation period. Conclusion A significant loss of viable CD34+ cells occurs during processing, cryopreservation and thawing. Low numbers of viable CD34+ cells infused post thaw will still result in adequate neutrophil engraftment however may delay platelet engraftment. Low viable CD34+ cell numbers have significant effects on admission duration and use of haematopoietic supportive measures with consequent effects on healthcare resources.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>24680293</pmid><doi>10.1016/j.transci.2014.02.021</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5219-5282</orcidid><orcidid>https://orcid.org/0000-0002-5983-753X</orcidid><orcidid>https://orcid.org/0000-0003-4837-5752</orcidid></addata></record>
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ispartof Transfusion and apheresis science, 2014-06, Vol.50 (3), p.443-450
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1878-1683
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source ScienceDirect Journals
subjects Adolescent
Adult
Aged
Amyloidosis - therapy
Antigens, CD34
Autografts
Autologous transplantation
CD34+ cell count
Cryopreservation
Female
Graft Survival
Health technology assessment
Hematology, Oncology and Palliative Medicine
Hematopoietic Stem Cells
Humans
Male
Middle Aged
Multiple Sclerosis - therapy
Neoplasms - therapy
Neutrophil engraftment
Neutrophils
Peripheral Blood Stem Cell Transplantation
Platelet engraftment
Retrospective Studies
title Pre infusion, post thaw CD34+ peripheral blood stem cell enumeration as a predictor of haematopoietic engraftment in autologous haematopoietic cell transplantation
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