Synthesis and Biological Activity of Vitamin D sub(3) 3 beta -Sulfate. Role of Vitamin D sub(3) Sulfates in Calcium Homeostasis

• Published in: The Journal of biological chemistry 1981-01, Vol.256 (11), p.5536-5539
• Main Authors: Kumar, R, Nagubandi, S, Londowski, J M, Bollman, S, Tietz, P
• Format: Article
• Language: English
• ISSN: 0021-9258

To determine the biological activity of vitamin D sulfates, the authors synthesized vitamin D sub(3) 3 beta -sulfate and tested its biological activity in vitamin D-deficient hypocalcemic rats. When vitamin D sub(3) sulfate was administered as a single oral dose of 208,000 or 416,000 pmol, it increased active calcium transport in the duodenum and was also able to mobilize calcium from bone and soft tissue. Vitamin D sub(3) itself was active at doses as low as 260 pmol when administered in this manner. In order to test the biological activity of vitamin D sub(3) sulfate in various doses when administered chronically, the authors tested the biological activity of vitamin D sub(3) sulfate after 5 days of oral dosing. When administered as a single intravenous dose, vitamin D sub(3) sulfate exhibited no biological activity in doses as high as 52,000 pmol. Vitamin D sub(3), however, was active at a dose of as low as 65 pmol. We conclude that vitamin D sub(3) sulfate, a metabolite of vitamin D sub(3) of heretofore unknown biological activity, is considerably less active than vitamin D sub(3) itself.