The use of an in vitro adventitious bud technique for mutation breeding of Begonia x hiëmalis

An in vitro propagation of two genotypes of Begonia x hienmalis leaf-disc explants and subsequent transplantation to soil of explant-parts with adventitious shoots. After irradiation of detached leaves with different doses of X-rays and two cycles of adventitious shoot formation on in vitro (sub)cul...

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Bibliographic Details
Published in:Euphytica 1981, Vol.30 (2), p.381-388
Main Authors: Roest, S., van Berkel, M. A. E., Bokelmann, G. S., Broertjes, C.
Format: Article
Language:English
Subjects:
Begonia
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Summary:An in vitro propagation of two genotypes of Begonia x hienmalis leaf-disc explants and subsequent transplantation to soil of explant-parts with adventitious shoots. After irradiation of detached leaves with different doses of X-rays and two cycles of adventitious shoot formation on in vitro (sub)cultured leaf-disc explants, plantlets were produced. About 30% of these plants was mutated with respect to e.g. the colour, size and form of the leaves and flowers. The great majority of the mutants (98.5%) proved to be solid (non-chimeric).
ISSN:0014-2336
1573-5060
DOI:10.1007/BF00034000