Detection of the Matrix Metalloproteinases MMP‐2 and MMP‐9 and Tissue Inhibitors of Metalloproteinases TIMP‐1 and TIMP‐2 in Llama (Lama glama) Oviduct

Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in several reproductive events like oocyte–spermatozoa interaction and semen liquefaction. In order to study their role in the llama oviductal reproductive process, MMP activity in oviductal fluid (OF) was assayed. Considerin...

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Bibliographic Details
Published in:Reproduction in domestic animals 2014-06, Vol.49 (3), p.492-498
Main Authors: Zampini, R, Argañaraz, ME, Miceli, DC, Apichela, SA
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal reproduction
Animals
Base Sequence
Camelids, New World
Cloning, Molecular
complementary DNA
Enzymes
Fallopian Tubes - chemistry
Fallopian Tubes - enzymology
Female
gelatin
gelatinase A
Gene Expression
llamas
Matrix Metalloproteinase 2 - analysis
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 9 - analysis
Matrix Metalloproteinase 9 - genetics
messenger RNA
Molecular Sequence Data
nucleotide sequences
RNA, Messenger - analysis
semen
Sequence Analysis, DNA
Tissue Inhibitor of Metalloproteinase-1 - analysis
Tissue Inhibitor of Metalloproteinase-1 - genetics
Tissue Inhibitor of Metalloproteinase-2 - analysis
Tissue Inhibitor of Metalloproteinase-2 - genetics
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Summary:Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in several reproductive events like oocyte–spermatozoa interaction and semen liquefaction. In order to study their role in the llama oviductal reproductive process, MMP activity in oviductal fluid (OF) was assayed. Considering that llama genome sequences are partially known, a strategy to procure cDNA sequences of MMP‐2, MMP‐9, TIMP‐1 and TIMP‐2 was designed. Afterwards, their expression patterns in the different llama oviductal segments were assayed. Gelatine zymograms detected 62 and 94 kDa protease activities that matched MMP‐2 and pro‐MMP‐9, respectively. Expression pattern analysis showed that MMP and TIMP mRNAs were present in ampulla, isthmus, utero‐tubal junction (UTJ) and papilla. Altogether, these findings support the argument that MMPs/TIMPs are produced in the oviduct and secreted into the oviductal lumen. Our results encourage further studies to elucidate the role of these proteins in reproductive oviductal events.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12317