Analysis of the endogenous human serum peptides by on-line extraction with restricted-access material and HPLC-MS/MS identification

The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP–...

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Bibliographic Details
Published in:Talanta (Oxford) 2014-09, Vol.127, p.191-195
Main Authors: Hu, Lianghai, Boos, Karl-Siegfried, Ye, Mingliang, Zou, Hanfa
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Humans
Ion Exchange
Molecular Weight
Online Systems
Peptides - blood
Peptides - chemistry
Proteomics
Restrict-access materials
Sample extraction
Serum - chemistry
Serum peptides
Silicon Dioxide
Tandem Mass Spectrometry
Two-dimensional
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Summary:The selective extraction of endogenous serum peptides has been a challenge due to the high abundant proteins present in serum. Here a simple on-line extraction of peptides from human serum using strong cation-exchange diol silica restricted-access materials (SCX-RAM) coupled with two-dimensional RP–RP liquid chromatography mass spectrometry was developed. The operation of the on-line extraction system is simple to use and does not need complex equipments. The two-dimensional RP–RP was proved to be orthogonal and efficient to separate peptides extracted from human serum. [Display omitted] •A simple system was established for on-line extraction of peptides from serum.•SCX-RAM materials were used for the selective enrichment of serum peptides.•2D-LC-MS was explored for the separation and identification of endogenous peptides.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2014.04.011