Specific oxygen uptake of the entomopathogenic nematode, Steinernema carpocapsae CABA01, in submerged culture

The specific oxygen uptake rate (qO 2 ) of stages of the entomopathogenic nematode (EPN) Steinernema carpocapsae CABA01 in liquid culture was measured. Nematodes were grown into previously pasteurised culture broths of their symbiotic bacterium, Xenorhabdus nematophila, in orbitally agitated flask c...

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Published in:Biocontrol science and technology 2014-07, Vol.24 (7), p.723-733
Main Authors: Chavarría-Hernández, Norberto, Pérez-Pérez, Nalleli-Concepción, Chavarría-Hernández, Juan-Carlos, Barahona-Pérez, Luis-Felipe, Rodríguez-Hernández, Adriana-Inés
Format: Article
Language:English
Subjects:
bioinsecticides
bioreactors
entomopathogenic nematodes
liquid culture
Nematoda
specific oxygen demands
Steinernema carpocapsae
Xenorhabdus nematophila
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Summary:The specific oxygen uptake rate (qO 2 ) of stages of the entomopathogenic nematode (EPN) Steinernema carpocapsae CABA01 in liquid culture was measured. Nematodes were grown into previously pasteurised culture broths of their symbiotic bacterium, Xenorhabdus nematophila, in orbitally agitated flask cultures (V L = 125 mL) at N = 150 rpm and T = 25°C. The basal medium contained 3% (w/v) soy trypticase broth and 0.5% (w/v) yeast extract. The EPNs developed from the egg stage to the adult stage exhibiting qO 2 values of 1.92, 5.48, 0.48, 0.28 and 0.0014 [10 −1 mmolO 2 /(g nematode-wet base h)] for the egg-Juvenile 1 (J1), J2, J3, J4 and the adult stages, respectively.
ISSN:0958-3157
1360-0478
DOI:10.1080/09583157.2014.890697