Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr

• Published in: Developmental and comparative immunology 2014-07, Vol.45 (1), p.133-140
• Main Authors: Philip, Anju M., Jørgensen, Even H., Maule, Alec G., Vijayan, Mathilakath M.
• Format: Article
• Language: English
• Subjects: Adiposity - immunology; Animals; Blood Glucose; Cytokines; Cytokines - genetics; Cytokines - metabolism; Fasting - physiology; Fish; Fish Proteins - genetics; Fish Proteins - metabolism; Gene Expression - immunology; Glycogen - metabolism; Hydrocortisone - blood; Immunity, Innate; Lipopolysaccharides - pharmacology; Liver - immunology; Liver - metabolism; Organ Specificity; Salmonidae - immunology; Salmonidae - metabolism; Salvelinus alpinus; Serum Amyloid A Protein - genetics; Serum Amyloid A Protein - metabolism; Serum amyloid protein A; SOCS; Spleen - metabolism; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; Suppressors of cytokine signalling
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2014.02.012

•LPS challenge upregulates IL-1b, IL-8 and SAA in the spleen and liver.•Extended fasting and emaciation does not curtail the ability to evoke an immune response to LPS challenge.•Extended fasting upregulates SOCS-1, SOCS-2 and SOCS-3 in a tissue-specific manner.•SOCS upregulation may be a key adaptation to restrict energy demanding pathways during extended fasting and emaciation. Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for 85days and challenged with lipopolysaccharide (LPS), and the molecular immune response in the liver and spleen assessed at 8 and 96h post-injection. LPS increased IL-1β, IL-8, and serum amyloid protein A (SAA) mRNA levels in both groups, but the liver IL-1β and IL-8, and spleen IL-8 responses were reduced in the fasted group. Fasting upregulated SOCS-1 and SOCS-2 mRNA abundance, while LPS stimulated SOCS-3 mRNA abundance and this response was higher in the fasted liver. Collectively, extended fasting and emaciation does not curtail the capacity of charr to evoke an immune response, whereas upregulation of SOCS may be a key adaptation to conserve energy by restricting the inflammatory response.