Ultrasonication assisted lipid extraction from oleaginous microorganisms

Various solvents, including water, hexane, methanol, and chloroform/methanol (1:1 v/v), were tested to identify the efficiency of lipid extraction from Trichosporon oleaginosus and an oleaginous fungal strain SKF-5 under ultrasonication (520 kHz 40 W and 50 Hz 2800 W) and compared with the conventio...

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Bibliographic Details
Published in:Bioresource technology 2014-04, Vol.158, p.253-261
Main Authors: XIAOLEI ZHANG, SONG YAN, TYAGI, Rajeshwar D, DROGUI, Patrick, SURAMPALLI, Rao Y
Format: Article
Language:English
Subjects:
Biofuel production
Biological and medical sciences
Biotechnology
Chloroform
Energy
Extraction
Fundamental and applied biological sciences. Psychology
Hexanes
Industrial applications and implications. Economical aspects
Lipids
Lipids - isolation & purification
Methyl alcohol
Microorganisms
Solvents - chemistry
Strain
Trichosporon - chemistry
Ultrasonic testing
Ultrasonics
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Summary:Various solvents, including water, hexane, methanol, and chloroform/methanol (1:1 v/v), were tested to identify the efficiency of lipid extraction from Trichosporon oleaginosus and an oleaginous fungal strain SKF-5 under ultrasonication (520 kHz 40 W and 50 Hz 2800 W) and compared with the conventional chloroform methanol (2:1 v/v) extraction method. The highest lipid recovery 10.2% and 9.3% with water, 43.2% and 33.2% with hexane, 75.7% and 65.1% with methanol, 100% and 100% w/w biomass with chloroform/methanol were obtained from T. oleaginosus and SKF-5 strain, respectively, at ultrasonication frequency 50 Hz and power input 2800 W. Ultrasonication chloroform/methanol extraction recovered total lipid in a short time (15 min) and low temperature (25°C). Whereas the conventional chloroform methanol extraction to achieve total lipid recovery required 12h at 60°C. Ultrasonication chloroform/methanol extraction would be a promising method of lipid extraction from the microorganisms.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2014.01.132