Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: An insight into the role of glial cells in retinal repair

• Published in: Experimental eye research 2013-07, Vol.112, p.68-78
• Main Authors: Machalińska, Anna, Kawa, Miłosz Piotr, Pius-Sadowska, Ewa, Rogińska, Dorota, Kłos, Patrycja, Baumert, Bartłomiej, Wiszniewska, Barbara, Machaliński, Bogusław
• Format: Article
• Language: English
• Subjects: Animals; Blotting, Western; Brain-Derived Neurotrophic Factor - genetics; Brain-Derived Neurotrophic Factor - metabolism; Cell Movement - drug effects; Cell Proliferation - drug effects; Electroretinography; Fluorescent Antibody Technique, Indirect; Gene Expression Profiling; Gene Expression Regulation - physiology; Glial Cell Line-Derived Neurotrophic Factor - genetics; Glial Cell Line-Derived Neurotrophic Factor - metabolism; glial cells; Iodates - toxicity; Mice; Nerve Growth Factors - genetics; Nerve Growth Factors - metabolism; Neuroglia - physiology; neurotrophins; Oligonucleotide Array Sequence Analysis; Proliferating Cell Nuclear Antigen - metabolism; Protein Array Analysis; regeneration; Regeneration - physiology; Retinal Degeneration - chemically induced; Retinal Degeneration - physiopathology; retinal injury; retinal pigment epithelium; Retinal Pigment Epithelium - drug effects; Retinal Pigment Epithelium - physiology; RNA, Messenger - metabolism; Signal Transduction; signaling pathways; sodium iodate
• ISSN: 0014-4835; 1096-0007
• DOI: 10.1016/j.exer.2013.04.004

The retinal pigment epithelium (RPE) has been reported to demonstrate feasible self-regenerative potential under specific conditions. However, the precise underlying mechanisms involved in this process are still elusive. Here, we performed a sequential morphological, molecular, and functional analysis of retinal injury and subsequent tissue regeneration after intravenous administration of a low dose of sodium iodate (15 mg/kg) in mice over long-term observation, up to 3 months post-injury. To assess the kinetics of the injury/recovery process, the electroretinography (ERG) responses were correlated with ongoing alterations in retinal structure and the global gene expression profile of injured retinas using genome-wide RNA microarray technology, western blotting and immunohistochemical analyses. We observed considerable improvement in the rod cell-mediated ERG response, which was accompanied by the regeneration of RPE within the injury site by the 3rd month post-injury. Our results confirm that the repairing mechanisms within injured retinas involve a significant glial cell reaction marked by glial cell proliferation, migration from their original location toward the injury site, followed by a significant overproduction of NTs such as BDNF, GDNF and NT-3. The global gene expression analysis revealed that initially up-regulated genes associated with cell death, apoptosis, acute response to stress pathways underwent considerable down-regulation in the late post-injury period. Accordingly, the genes implicated in nervous tissue remodeling and neuron development, the regulation of synaptic transmission and the establishment of localization were substantially induced by the 3rd month. Collectively, our observations support the view that Müller glial cells might well play an active role not only in retinal cell reorganization following injury but potentially also in RPE regeneration, which appears to be the key event in retinal reparative process. Furthermore, we provided novel compelling evidence of the crucial role of neurotrophins in the pathophysiology of retinal repair and identified the signaling pathways that are activated during this process. •RPE regenerate at the sites of injury after 3 months post sodium iodate injection.•The repairing mechanisms within injured retinas involve a glial cell activation.•Endogenous neurotrophic factors and their receptors play a role in the regeneration.•Alterations in gene expression occur during both retinal damage and