Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2 O2 Induced Toxicity on Dental Pulp Cells

• Published in: BioMed research international 2014-01, Vol.2014
• Main Authors: Vargas, Fernanda da Silveira, Soares, Diana Gabriela, Ribeiro, Ana Paula Dias, Hebling, Josimeri, De Souza Costa, Carlos Alberto
• Format: Article
• Language: English
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/895049

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol ( alpha -T) isomer against the toxicity of hydrogen peroxide (H sub(2) O sub(2) ) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM alpha -T for 60 minutes. They were then exposed or not to H sub(2) O sub(2) for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H sub(2) O sub(2) (0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; alpha =5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated with alpha -T at 1, 3, 5, and 10 mM concentrations and exposed to H sub(2) O sub(2) had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect of alpha -T against the H sub(2) O sub(2) toxicity. Overall, the vitamin E alpha -T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H sub(2) O sub(2) . The most effective cell protection was provided by 5 and 10 mM concentrations of alpha -T.