Detection of enterovirus genome sequence from diarrheal feces of goat

Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5′-untranslated region, and we determined 563 b...

Full description

Saved in:
Bibliographic Details
Published in:Virus genes 2014-06, Vol.48 (3), p.550-552
Main Authors: Omatsu, Tsutomu, Tsuchiaka, Shinobu, Hirata, Teppei, Shiroma, Yasushi, Okazaki, Sachiko, Katayama, Yukie, Oba, Mami, Nishiura, Naomi, Sassa, Yukiko, Furuya, Tetsuya, Nagai, Makoto, Ochiai, Hideharu, Tamaki, Shirou, Mizutani, Tetsuya
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biomedical and Life Sciences
Biomedicine
Cattle
Diarrhea - veterinary
Diarrhea - virology
Enterovirus
Enterovirus - classification
Enterovirus - genetics
Enterovirus - isolation & purification
feces
Feces - virology
Genome, Viral
Goat Diseases - virology
Goats
Medical Microbiology
Molecular Sequence Data
nucleotide sequences
Phylogeny
Plant Sciences
reverse transcriptase polymerase chain reaction
Sheep
Virology
viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5′-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-014-1057-9