PCR detection of Neospora caninum in water buffalo foetal tissues

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission ca...

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Bibliographic Details
Published in:Acta parasitologica 2014-03, Vol.59 (1), p.1-4
Main Authors: Auriemma, Clementina, Lucibelli, Maria Gabriella, Borriello, Giorgia, De Carlo, Esterina, Martucciello, Alessandra, Schiavo, Lorena, Gallo, Amalia, Bove, Francesca, Corrado, Federica, Girardi, Santa, Amoroso, Maria Grazia, Ďegli Uberti, Barbara, Galiero, Giorgio
Format: Article
Language:English
Subjects:
Abortion
Abortion, Veterinary - epidemiology
Abortion, Veterinary - parasitology
Animal Systematics/Taxonomy/Biogeography
Animals
Biomedical and Life Sciences
Biomedicine
Brain
Buffalo
Buffaloes
Cattle
Coccidiosis - epidemiology
Coccidiosis - parasitology
Coccidiosis - veterinary
Dairy farms
Ecology
Female
Fetus - parasitology
Heart
Italy - epidemiology
Medical Microbiology
Microbiology
Neospora - genetics
Neospora - isolation & purification
Neospora caninum
Original Paper
Parasites
Parasitology
PCR
Phylogenetics
Phylogeny
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
Pregnancy
Prevalence
Serology
water buffalo
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Summary:The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.
ISSN:1230-2821
1896-1851
1896-1851
DOI:10.2478/s11686-014-0201-y