MicroRNA-30c serves as an independent biochemical recurrence predictor and potential tumor suppressor for prostate cancer

• Published in: Molecular biology reports 2014-05, Vol.41 (5), p.2779-2788
• Main Authors: Ling, Xiao-hui, Han, Zhao-dong, Xia, Dan, He, Hui-chan, Jiang, Fu-neng, Lin, Zhuo-yuan, Fu, Xin, Deng, Ye-han, Dai, Qi-shan, Cai, Chao, Chen, Jia-hong, Liang, Yu-xiang, Zhong, Wei-de, Wu, Chin-lee
• Format: Article
• Language: English
• Subjects: Adult; Aged; Animal Anatomy; Animal Biochemistry; Biochemistry; Biomedical and Life Sciences; carcinogenesis; Cell Movement - genetics; Cell Proliferation; Disease Progression; Gene Expression Regulation, Neoplastic; Genes, Tumor Suppressor; Histology; Humans; Life Sciences; Male; Medical prognosis; MicroRNAs; MicroRNAs - genetics; Middle Aged; Morphology; multivariate analysis; Neoplasm Grading; Neoplasm Staging; patients; Prognosis; Prostate cancer; Prostate-Specific Antigen - metabolism; prostatic neoplasms; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - mortality; Prostatic Neoplasms - pathology; Prostatic Neoplasms - surgery; Recurrence; reverse transcriptase polymerase chain reaction; ROC Curve
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3132-7

MicroRNA-30c (miR-30c) acts as a tumor suppressor or a tumor promoter in various human malignancies. However, the involvement of miR-30c in prostate cancer (PCa) is still unclear. The aim of this study was to investigate the molecular function and the clinical significance of miR-30c in PCa. Expression levels of miR-30c in PCa tissues and cells were detected by quantitative real-time-PCR (qRT-PCR). Additionally, the associations of miR-30c expression with clinicopathological features and prognosis in PCa patients were analyzed. The potential role of miR-30c in tumorigenesis of PCa cells was further evaluated by in vitro cell assays. MiR-30c was significantly down-regulated in PCa tissues and cells compared with the corresponding controls (P < 0.05). In addition, the downregulation of miR-30c in PCa tissues was significantly associated with higher Gleason score (P = 0.009), advanced pathological stage (P = 0.016) and biochemical recurrence (P = 0.034). Moreover, Kaplan–Meier survival analysis showed that the reduced expression of miR-30c was correlated with shorter biochemical recurrence-free survival (P = 0.023). The multivariate analysis also identified miR-30c as an independent prognostic predictor for biochemical recurrence-free survival in patients with PCa. Furthermore, the enforced expression of miR-30c suppressed proliferation, migration and invasion of PCa cells in vitro. Our data indicated the involvement of miR-30c in PCa progression and suggested its potential role as an independent predictor of biochemical recurrence in PCa. On cellular level, miR-30c may function as a tumor suppressor for PCa cells by inhibiting tumor cell proliferation, migration and invasion.