A universal primer set to amplify the cytochrome c oxidase subunit I gene in bears

Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available...

Full description

Saved in:
Bibliographic Details
Published in:Ursus (International Association for Bear Research and Management) 2014-05, Vol.25 (1), p.73-77
Main Authors: Shahzadi, Iram, Janjua, Safia, Fakhar-i-Abbas, Galbreath, Gary J
Format: Article
Language:English
Subjects:
Asiatic black bear
cytochrome c oxidase subunit I (COI) gene
DNA barcoding
Himalayan brown bear
Short Communications
Ursidae
Ursus
Ursus arctos
Ursus thibetanus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n  =  12) and Himalayan brown (U. arctos; n  =  6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83–92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.
ISSN:1537-6176
1938-5439
DOI:10.2192/URSUS-D-13-00014.1